Mycobacterium tuberculosis WhiB3 maintains redox homeostasis and survival in response to reactive oxygen and nitrogen species.
Mansi Mehta,Amit Singh +1 more
TLDR
Empirical proof is provided that WhiB3 is required to mitigate redox stress induced by ROS and RNS, which may be important to activate host/bacterial pathways required for the granuloma development and maintenance.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2019-02-01 and is currently open access. It has received 43 citations till now. The article focuses on the topics: Reactive nitrogen species & Reactive oxygen species.read more
Citations
More filters
Journal ArticleDOI
Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis.
Neharika Jain,Haroon Kalam,Lakshya Veer Singh,Vartika Sharma,Saurabh Kedia,Prasenjit Das,Vineet Ahuja,Dhiraj Kumar +7 more
TL;DR: It is shown that mesenchymal stem cells can harbor Mycobacterium tuberculosis providing a niche for evasion of anti-bacterial drugs and cytokines, which can have a major impact on tuberculosis prevention and cure.
Journal ArticleDOI
Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112.
Kawther Alqaseer,Kawther Alqaseer,Obolbek Turapov,Philippe Barthe,Heena Jagatia,Angelique De Visch,Christian Roumestand,Malgorzata Wegrzyn,Iona L. Bartek,Martin I. Voskuil,Helen M. O'Hare,Paul Ajuh,Andrew R. Bottrill,Adam A. Witney,Martin Cohen-Gonsaud,Simon J. Waddell,Galina V. Mukamolova +16 more
TL;DR: It is observed that depletion of PknB resulted in specific transcriptional changes that are likely caused by reduced phosphorylation of the H‐NS‐like regulator LSR2 at threonine 112, which controls Mtb growth and adaptations to the changing host environment by phosphorylating the global transcriptional regulator Lsr2.
Journal ArticleDOI
Mycobacterium tuberculosis SufR responds to nitric oxide via its 4Fe-4S cluster and regulates Fe-S cluster biogenesis for persistence in mice.
Kushi Anand,Ashutosh Tripathi,Kaustubh Shukla,Nitish Malhotra,Anil Kumar Jamithireddy,Rajiv Kumar Jha,Susmit Narayan Chaudhury,R. S. Rajmani,Arati Ramesh,Valakunja Nagaraja,Balasubramanian Gopal,Ganesh Nagaraju,Aswin Sai Narain Seshayee,Amit Singh +13 more
TL;DR: In this article, a transcription factor SufR (Rv1460) senses NO via its 4Fe-4S cluster and promotes persistence of Mycobacterium tuberculosis (Mtb) by mobilizing the Fe-S cluster biogenesis system.
Journal ArticleDOI
Recent Developments in the Application of Flow Cytometry to Advance our Understanding of Mycobacterium tuberculosis Physiology and Pathogenesis
TL;DR: Current knowledge of flow cytometry applications that have advanced the authors' understanding of the physiology of M. tuberculosis during TB disease are summarized and it is highlighted that flow cytometers offers unprecedented opportunities for insight into bacterial population heterogeneity, which is increasingly appreciated as an important determinant of disease outcome.
References
More filters
Journal ArticleDOI
Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells
Dennis J. Stuehr,Carl Nathan +1 more
TL;DR: M phi-generated NO.
Journal ArticleDOI
Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase
Sheila Sturgill-Koszycki,Paul H. Schlesinger,Prasanta Chakraborty,Pryce L. Haddix,Helen L. Collins,Agnes K. Fok,Richard D. Allen,Stephen L. Gluck,John E. Heuser,David G. Russell +9 more
TL;DR: Immunoelectron microscopy of infected macrophages and immunoblotting of isolated phagosomes showed that Mycobacterium vacuoles acquire the lysosomal membrane protein LAMP-1, but not the vesicular proton-adenosine triphosphatase (ATPase) responsible for phagosomal acidification.
Journal ArticleDOI
Nitrosylation. the prototypic redox-based signaling mechanism.
TL;DR: Whereas phosphorylation clearly Spain lies at the heart of many signal transduction pathways, has been expanded re-translational modification of proteins, are conserved cently by the discovery of an enzymatic function for throughout evolution and influence most aspects of cel-hemoglobin.
Journal ArticleDOI
Identification of nitric oxide synthase as a protective locus against tuberculosis
TL;DR: NOS2(-/-) mice proved highly susceptible, resembling wild-type littermates immunosuppressed by high-dose glucocorticoids, and allowed Mycobacterium tuberculosis to replicate faster in the lungs than reported for other gene-deficient hosts.
Journal ArticleDOI
The role of the granuloma in expansion and dissemination of early tuberculous infection.
TL;DR: In this paper, the authors use quantitative intravital microscopy to reveal distinct steps of granuloma formation and assess their consequence for infection, showing that pathogenic mycobacteria exploit the granulomas during the innate immune phase for local expansion and systemic dissemination.