Nanoscale analysis reveals agonist-sensitive and heterogeneous pools of phosphatidylinositol 4-phosphate in the plasma membrane.
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TLDR
The results suggest that the level of the PtdIns(4)P pool in the plasma membrane is sensitive and the distribution of PTDIns( 4)P dramatically changes by agonist stimulation, and there are active sites of production or replenishment of Ptd insurance at undifferentiated membrane and caveolar areas.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2016-06-30 and is currently open access. It has received 14 citations till now. The article focuses on the topics: Phosphatidylinositol 4-phosphate & Phosphatidylinositol.read more
Citations
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Journal ArticleDOI
Osh Proteins Control Nanoscale Lipid Organization Necessary for PI(4,5)P2 Synthesis
Taki Nishimura,Michael Gecht,Roberto Covino,Gerhard Hummer,Michal A. Surma,Christian Klose,Hiroyuki Arai,Nozomu Kono,Christopher J. Stefan +8 more
TL;DR: It is shown that ORP-related Osh lipid exchange proteins are critical for the synthesis of phosphatidylinositol (4,5)-bisphosphate [PI( 4,5)P2], a key regulator of dynamic events at the PM.
Journal ArticleDOI
In focus in HCB.
Douglas J. Taatjes,Jürgen Roth +1 more
TL;DR: Taking all of the data into consideration, it is recommended that DNase I and citrate buffer may offer less harsh alternatives to hydrochloric acid for DNA denaturation in the BrdU staining protocol.
Journal ArticleDOI
Liposomes as colloidal nanovehicles: on the road to success in intravenous drug delivery
TL;DR: The first closed bilayer phospholipid system, the liposome system, has been making steady progress over five decades of extensive research and has been efficient in achieving many desirable parameters such as remote drug loading, size-controlling measures, longer circulation half-lives, and triggered release.
Journal ArticleDOI
Nanoscale domain formation of phosphatidylinositol 4-phosphate in the plasma and vacuolar membranes of living yeast cells.
Kanna Tomioku,Mikiko Shigekuni,Hiroki Hayashi,Akane Yoshida,Taiki Futagami,Hisanori Tamaki,Kenji Tanabe,Akikazu Fujita +7 more
TL;DR: The present study showed that PtdIns(4)P is specifically localised in the flat undifferentiated plasma membrane compartment and in the vacuolar membrane, where it was concentrated in intramembrane particle (IMP)-deficient raft-like domains, which are tightly bound to lipid droplets, but not surrounding IMP-rich non-raft domains in geometrical IMP-distributed patterns in the stationary phase.
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Phosphatidylinositol 4-phosphate on Rab7-positive autophagosomes revealed by the freeze-fracture replica labeling.
Yuna Kurokawa,Akane Yoshida,Emi Fujii,Kanna Tomioku,Hiroki Hayashi,Kenji Tanabe,Akikazu Fujita +6 more
TL;DR: It is suggested that PtdIns(4)P is localized to the cytoplasmic leaflet of the autophagosome at later stages, which may illuminate the importance of Ptd insurance at the later stages of autophosome formation.
References
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Journal ArticleDOI
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation
TL;DR: This review is an attempt to give an overview of this enormous research field focusing on major developments in diverse areas of basic science linked to cellular physiology and disease.
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Direct visualization of ras proteins in spatially distinct cell surface microdomains
TL;DR: It is found that an inner-plasma membrane lipid raft marker displays cholesterol-dependent clustering in microdomains with a mean diameter of 44 nm that occupy 35% of the cell surface, illustrating that the inner plasma membrane comprises a complex mosaic of discrete micro domains.
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PIP2 is a necessary cofactor for ion channel function: how and why?
Byung-Chang Suh,Bertil Hille +1 more
TL;DR: This review discusses the dependence of ion channels on phosphoinositides and considers possible mechanisms by which PIP2 and analogues regulate ion channel activity.
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Membrane Phospholipid Control of Nucleotide Sensitivity of KATP Channels
Show Ling Shyng,Colin G. Nichols +1 more
TL;DR: It is proposed that membrane-incorporated PIPs can bind to positive charges in the cytoplasmic region of the KATP channel's Kir6.2 subunit, stabilizing the open state of the channel and antagonizing the inhibitory effect of ATP.
Journal ArticleDOI
PIP2 and PIP as Determinants for ATP Inhibition of KATP Channels
Thomas Baukrowitz,Uwe Schulte,Dominik Oliver,Stefan Herlitze,Tobias Krauter,Stephen J. Tucker,J. Peter Ruppersberg,Bernd Fakler +7 more
TL;DR: It is reported here that phosphatidylinositol-4, 5-bisphosphate (PIP2) and phosphorus-4-phosphates(PIP) controlled ATP inhibition of cloned KATP channels (Kir6.2 and SUR1) and represents a mechanism for control of excitability through phospholipids.