New insights into RNA secondary structure in the alternative splicing of pre-mRNAs.
Yongfeng Jin,Yun Yang,Peng Zhang +2 more
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TLDR
Several recent discoveries related to the role of RNA secondary structure in alternative splicing and the underlying mechanisms are reviewed, including an RNA pairing-based mechanism that ensures the selection of only one of several available exons.Abstract:
Alternative splicing is an important mechanism in generating proteomic diversity, and RNA secondary structure is an important element in splicing regulation. The use of high-throughput sequencing and other approaches has increased the number of known pre-mRNA secondary structures by several orders of magnitude, and we now have new insights into the role of RNA secondary structure in alternative splicing and the mechanisms involved (e.g., physical competition, long-range RNA pairing, the structural splicing code, and co-transcriptional splicing). Furthermore, an RNA pairing-based mechanism ensures the selection of only one of several available exons (e.g., Dscam splicing). Here we review several recent discoveries related to the role of RNA secondary structure in alternative splicing and the underlying mechanisms.read more
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In vivo genome-wide profiling of RNA secondary structure reveals novel regulatory features
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Complexity of the Alternative Splicing Landscape in Plants
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The multiple functions of RNA helicases as drivers and regulators of gene expression
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TL;DR: The spliceosome exhibits exceptional compositional and structural dynamics that are exploited during substrate-dependent complex assembly, catalytic activation, and active site remodeling in the pre-mRNAs.
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TL;DR: It is now clear that the 'missing' information is in large part provided by alternative splicing, the process by which multiple different functional messenger RNAs, and therefore proteins, can be synthesized from a single gene.