Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016.
Alessandra Carattoli,Laura Villa,Claudia Feudi,Claudia Feudi,Ludovica Curcio,Serenella Orsini,Andrea Luppi,Giovanni Pezzotti,Chiara Francesca Magistrali +8 more
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TLDR
Immediate implementation of mcr-screening including this novel gene variant is required for Salmonella and E. coli from humans and food-producing animals in Europe.Abstract:
A novel mcr colistin resistance gene was identified in a strain of Salmonella enterica, monophasic variant of serovar Typhimurium (4,5,12:i:- ), isolated from a pig at slaughter in Italy in 2013, and in Escherichia coli strains collected during routine diagnostic of post-weaning diarrhoea in pigs from Spain and Belgium in 2015 and 2016. Immediate implementation of mcr-screening including this novel gene variant is required for Salmonella and E. coli from humans and food-producing animals in Europe.read more
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Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae
Marta Rozwandowicz,Michael S.M. Brouwer,Jennie Fischer,Jaap A. Wagenaar,Bruno Gonzalez-Zorn,Beatriz Guerra,Dik Mevius,Joost Hordijk +7 more
TL;DR: The purpose of this review is to provide an overview of all known AMR-related plasmid families in Enterobacteriaceae, the resistance genes they carry and their geographical distribution.
Journal ArticleDOI
The global distribution and spread of the mobilized colistin resistance gene mcr-1.
Ruobing Wang,Lucy van Dorp,Liam P. Shaw,Phelim Bradley,Qi Wang,Xiaojuan Wang,Longyang Jin,Zhang Qing,Yuqing Liu,Adrien Rieux,Thamarai Dorai-Schneiders,Lucy A. Weinert,Zamin Iqbal,Zamin Iqbal,Xavier Didelot,Hui Wang,Francois Balloux +16 more
TL;DR: The results provide the first systematic phylogenetic analysis of the origin and spread of mcr-1, and emphasize the importance of understanding the movement of antibiotic resistance genes across multiple levels of genomic organization.
Journal ArticleDOI
Emergence of a novel mobile colistin resistance gene, mcr-8, in NDM-producing Klebsiella pneumoniae.
Xiaoming Wang,Yao Wang,Ying Zhou,Jiyun Li,Wenjuan Yin,Shaolin Wang,Suxia Zhang,Jianzhong Shen,Zhangqi Shen,Yang Wang +9 more
TL;DR: Functional cloning indicated that the acquisition of the single mcr-8 gene significantly increased resistance to colistin in both Escherichia coli and K. pneumoniae.
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Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B.
Maria Borowiak,Jennie Fischer,Jens A. Hammerl,Rene S. Hendriksen,Istvan Szabo,Burkhard Malorny +5 more
TL;DR: The findings suggest that the transfer of colistin-resistance-mediating phosphoethanolamine transferase genes from bacterial chromosomes to mobile genetic elements has occurred in multiple independent events raising concern regarding their variety, prevalence and impact on public health.
Journal ArticleDOI
Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes.
Ana Rita Rebelo,Valeria Bortolaia,Jette Kjeldgaard,Susanne Karlsmose Pedersen,Pimlapas Leekitcharoenphon,Inge M Hansen,Beatriz Guerra,Burkhard Malorny,Maria Borowiak,Jens A. Hammerl,Antonio Battisti,Alessia Franco,Patricia Alba,Agnès Perrin-Guyomard,Sophie A. Granier,Cristina De Frutos Escobar,Surbhi Malhotra-Kumar,Laura Villa,Alessandra Carattoli,Rene S. Hendriksen +19 more
TL;DR: The multiplex PCR protocol should be particularly interesting in settings or laboratories with limited resources for performing genetic analysis as it provides information on the mechanism of colistin resistance without requiring genome sequencing.
References
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Matthew Kearse,Richard Moir,Amy Wilson,Steven Stones-Havas,Matthew Cheung,Shane Sturrock,Simon Buxton,Alex Cooper,Sidney Markowitz,Chris Duran,Tobias Thierer,Bruce Ashton,Peter Meintjes,Alexei J. Drummond +13 more
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TL;DR: A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.
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Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study
Yiyun Liu,Yang Wang,Timothy R. Walsh,Ling-Xian Yi,Rong Zhang,James Spencer,Yohei Doi,Guo-Bao Tian,Baolei Dong,Xianhui Huang,Lin-Feng Yu,Danxia Gu,Hongwei Ren,Xiaojie Chen,Luchao Lv,Dandan He,Hongwei Zhou,Zi-sen Liang,Jian-Hua Liu,Jianzhong Shen +19 more
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Journal ArticleDOI
In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing
Alessandra Carattoli,Ea Zankari,Aurora García-Fernández,Mette Voldby Larsen,Ole Lund,Laura Villa,Frank Møller Aarestrup,Henrik Hasman +7 more
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Polymyxins: Antibacterial Activity, Susceptibility Testing, and Resistance Mechanisms Encoded by Plasmids or Chromosomes
TL;DR: Genotypic and phenotypic methods that provide relevant information for diagnostic laboratories are presented and recent works in relation to recently identified mechanisms of polymyxin resistance, including chromosomally encoded resistance traits as well as the recently identified plasmid-encoded polymyXin resistance determinant MCR-1 are presented.