Oral Somatic Transgene Vaccination Using Attenuated S. typhimurium
Ayub Darji,Carlos A. Guzmán,Birgit Gerstel,Petra Wachholz,Kenneth N. Timmis,Jürgen Wehland,Trinad Chakraborty,Siegfried Weiss +7 more
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TLDR
A highly versatile system for antigen delivery, identification of protective antigens for vaccination, and efficient generation of antibodies against the product of open reading frames present on virtually any DNA segment is described.About:
This article is published in Cell.The article was published on 1997-12-12 and is currently open access. It has received 446 citations till now. The article focuses on the topics: Bacterial vaccine & Aroa.read more
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DNA vaccines: immunology, application, and optimization*.
TL;DR: This review focuses on the mechanisms by which DNA vaccines elicit immune responses, and a list of potential applications in a variety of preclinical models is provided.
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Engineering the perfect (bacterial) cancer therapy
TL;DR: It is proposed that synthetic biology techniques can be used to solve many of the key challenges that are associated with bacterial therapies, such as toxicity, stability and efficiency, and can been used to tune their beneficial features, allowing the engineering of 'perfect' cancer therapies.
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A DNA vaccine against VEGF receptor 2 prevents effective angiogenesis and inhibits tumor growth.
Andreas G. Niethammer,Rong Xiang,Jürgen C. Becker,Harald Wodrich,Ursula Pertl,Gabriele Karsten,Brian P. Eliceiri,Ralph A. Reisfeld +7 more
TL;DR: This vaccine effectively protected mice from lethal challenges with melanoma, colon carcinoma and lung carcinoma cells and reduced growth of established metastases in a therapeutic setting and may provide a new strategy for the rational design of cancer therapies.
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Spinning molecular immunology into successful immunotherapy.
TL;DR: Some of the most promising molecular and cellular targets for immunotherapy are reviewed and approaches that use these targets to amplify immune responses and potentially break antigen-specific tolerance are discussed, providing a blueprint for the development of successful immunotherapy over the next decade.
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Tumour-targeting bacteria engineered to fight cancer
TL;DR: Therapy with live tumour-targeting bacteria represents a unique opportunity to address the limitations associated with molecularly targeted therapies and immunotherapies and outline advances in the engineering of bacteria, which have the potential to improve safety and efficacy.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
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TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
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Cpg motifs in bacterial dna trigger direct b-cell activation
Arthur M. Krieg,Ae-Kyung Yi,Sara Matson,Thomas J. Waldschmidt,Gail A. Bishop,Gail A. Bishop,Rebecca M. Teasdale,Gary A. Koretzky,Dennis M. Klinman +8 more
TL;DR: The potent immune activation by CpG oligon nucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.