Patterns of matrix metalloproteinase expression in cycling endometrium imply differential functions and regulation by steroid hormones.
William H. Rodgers,Lynn M. Matrisian,Linda C. Giudice,B A Dsupin,P. Cannon,C. Svitek,Fred Gorstein,Kevin G. Osteen +7 more
TLDR
Data indicate that matrix metalloproteinases are expressed in cell-type, tissue, and reproductive cycle-specific patterns, consistent with regulation by steroid hormones, and with specific roles in the complex tissue growth and remodeling processes occurring in the endometrium during the reproductive cycle.Abstract:
Matrix metalloproteinases are a highly regulated family of enzymes, that together can degrade most components of the extracellular matrix. These proteins are active in normal and pathological processes involving tissue remodeling; however, their sites of synthesis and specific roles are poorly understood. Using in situ hybridization, we determined cellular distributions of matrix metalloproteinases and tissue inhibitor of metalloproteinase-1, an inhibitor of matrix metalloproteinases, in endometrium during the reproductive cycle. The mRNAs for all the metalloproteinases were detected in menstrual endometrium, but with different tissue distributions. The mRNA for matrilysin was localized to epithelium, while the others were detected in stromal cells. Only the transcripts for the 72-kD gelatinase and tissue inhibitor of metalloproteinases-1 were detected throughout the cycle. Transcripts for stromelysin-2 and the 92-kD gelatinase were only detected in late secretory and menstrual endometrium, while those for matrilysin, the 72-kD gelatinase, and stromelysin-3 were also consistently detected in proliferative endometrium. These data indicate that matrix metalloproteinases are expressed in cell-type, tissue, and reproductive cycle-specific patterns, consistent with regulation by steroid hormones, and with specific roles in the complex tissue growth and remodeling processes occurring in the endometrium during the reproductive cycle.read more
Citations
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Proteolytic remodeling of extracellular matrix
TL;DR: Evidence is emerging that these enzymes also play an important regulatory role in matrix remodeling by catalyzing the processing of inactive matrix metalloproteinase and cytokine precursors.
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Pathogenesis and pathophysiology of endometriosis
TL;DR: The disease process is reviewed from theories regarding origin to the molecular basis for disease sequelae and a thorough understanding of the histopathogenesis and pathophysiology of endometriosis is essential to the development of novel diagnostic and treatment approaches for this debilitating condition.
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Endometriosis: epidemiology and aetiological factors
TL;DR: These epidemiological findings strongly support the menstrual reflux hypothesis and include the demonstration of viable endometrial cells in the menstrual effluent and peritoneal fluid, and an association between obstructed menstrual outflow and endometriosis.
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The Matrix Metalloproteinase System: Changes, Regulation, and Impact throughout the Ovarian and Uterine Reproductive Cycle
Thomas E. Curry,Kevin G. Osteen +1 more
TL;DR: The current review will highlight the key features of the MMPs and tissue inhibitors of metalloproteinases, focus on the changes and regulation of theMMP system that take place throughout the estrous and menstrual cycles, and address the impact of the dynamic tissue remodeling processes on ovarian and uterine physiology.
References
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Matrix metalloproteinases and their inhibitors in connective tissue remodeling.
TL;DR: Latency is overcome by physical, chemical, and enzymatic treatments that separate the cysteine residue from the zinc Expression of the metalloproteinases is switched on by a variety of agents acting through regulatory elements of the gene, particularly the AP‐1 binding site.
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Dating the endometrial biopsy.
TL;DR: It is asserted that examination of the endometrium during the secretory phase yields more information about the time of ovulation degree of progestational change and normality of theendometrium than any other test used in sterility studies.
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Matrix Metalloproteinases: A Review
Henning Birkedal-Hansen,William G. I. Moore,M.K. Bodden,L.J. Windsor,B. Birkedal-Hansen,Arthur A. Decarlo,Jeffrey A. Engler +6 more
TL;DR: The present review discusses in detail the primary structures and the overlapping yet distinct substrate specificities of MMPs as well as the mode of activation of the unique MMP precursors.
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Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes
TL;DR: Estimates from the observed signals indicate that a large fraction of target RNAs is both retained in sections and hybridized with probe at saturation, and coupled with measurements of nonspecific background binding of heterologous probes, these data indicate that the method has sufficient sensitivity to detect many moderately abundant mRNAs.
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Antitumor promotion and antiinflammation: Down-modulation of AP-1 (Fos/Jun) activity by glucocorticoid hormone
Carsten Jonat,Hans J. Rahmsdorf,Kun-Koo Park,Andrew C.B. Cato,Stephan Gebel,Helmut Ponta,Peter Herrlich +6 more
TL;DR: Coprecipitation experiments suggest direct AP-1-hormone receptor interaction, which also possibly explains the reverse experiment: overexpression of Fos or Jun inhibits the expression of hormone-dependent genes.