Journal ArticleDOI
Production of Fumaric Acid by Bioconversion of Corncob Hydrolytes Using an Improved Rhizopus oryzae Strain
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The use of a furfural-resistant strain developed through domestication effectively increased the titer of FA and this capacity of the microorganisms to produce high amounts of FA by bioconverting corncob hydrolyte can be further applied for industrial production of FA.Abstract:
The use of microorganism fermentation for production of fumaric acid (FA), which is widely used in food, medicine, and other fields, can provide technical support for the FA industry. In this study, we aimed to increase the titer of FA production by using an improved Rhizopus oryzae WHT5, which was domesticated to obtain a furfural-resistant strain in corncob hydrolytes. The metabolic pathways and metabolic network of this strain were investigated, and the related enzymes and metabolic flux were analyzed. Metabolic pathway analysis showed that the R. oryzae WHT5 strain produced FA mainly through two pathways. One occurred in the cytoplasm and the other was a mitochondrial pathway. The key parameters of the fermentation process were analyzed. The FA titer was 49.05 g/L from corncob hydrolytes using R. oryzae WHT5 in a 7-L bioreactor. The use of a furfural-resistant strain developed through domestication effectively increased the titer of FA. This capacity of the microorganisms to produce high amounts of FA by bioconverting corncob hydrolyte can be further applied for industrial production of FA.read more
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Notes for genera: basal clades of Fungi (including Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota)
Nalin N. Wijayawardene,Nalin N. Wijayawardene,Julia Pawłowska,Peter M. Letcher,Paul M. Kirk,Richard A. Humber,Arthur Schüßler,Marta Wrzosek,Anna Muszewska,Alicja Okrasińska,Łukasz Istel,Aleksandra Gęsiorska,Paul G. Mungai,A. A. Lateef,Kunhiraman C. Rajeshkumar,Rajshree V. Singh,Renate Radek,Grit Walther,Lysett Wagner,Christopher Walker,Christopher Walker,D. Siril Abeywickrama Wijesundara,Moslem Papizadeh,Somayeh Dolatabadi,Belle Damodara Shenoy,Yuri Tokarev,Saisamorn Lumyong,Kevin D. Hyde,Kevin D. Hyde +28 more
TL;DR: 611 genera in 153 families, 43 orders and 18 classes are provided with details of classification, synonyms, life modes, distribution, recent literature and genomic data.
Journal ArticleDOI
Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation
TL;DR: Solid state fermentation of raw oil palm frond leaves as the substrate to produce extracellular cellulases and xylanase by a novel Rhizopus oryzae UC2 supported its prospective biocatalytic role for timely and safe production of digestible carbohydrates from agro-industrial biomass for the subsequent biotransformation into biofuel.
Journal ArticleDOI
Fungal-Assisted Valorization of Raw Oil Palm Leaves for Production of Cellulase and Xylanase in Solid State Fermentation Media
Uchenna R. Ezeilo,Roswanira Abdul Wahab,Lee Chew Tin,Iffah Izzati Zakaria,Fahrul Huyop,Naji Arafat Mahat +5 more
TL;DR: The approach adopted by this study offers an alternative avenue to valorizing agriculture biomass, in conjunction to sustainably produce cellulose-acting enzymes to catalyse biofuel and platform chemical productions, yielding satisfactory high titers of cellulase and xylanase.
Journal ArticleDOI
Four-carbon dicarboxylic acid production through the reductive branch of the open cyanobacterial tricarboxylic acid cycle in Synechocystis sp. PCC 6803.
Hiroko Iijima,Atsuko Watanabe,Haruna Sukigara,Kaori Iwazumi,Tomokazu Shirai,Akihiko Kondo,Takashi Osanai +6 more
TL;DR: In this article, the authors report C4 dicarboxylic acid production from CO2 using metabolically engineered Synechocystis sp. PCC 6803.
Journal ArticleDOI
Efficient whole‐cell biotransformation of furfural to furfuryl alcohol by Saccharomyces cerevisiae NL22
TL;DR: The bioprocess developed in this study is a highly efficient method for FOL synthesis and the wild‐type strain S. cerevisiae NL22 might serve as a candidate for the production of FAL to FOL as an efficient biocatalyst.
References
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Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae
Sandra A Allen,William Clark,J. Michael McCaffery,Zhen Cai,Alison Lanctot,Patricia J. Slininger,Z. Lewis Liu,Steven W. Gorsich +7 more
TL;DR: It is demonstrated that furfural induces the accumulation of ROS in Saccharomyces cerevisiae and was shown to cause cellular damage that is consistent with ROS accumulation in cells which includes damage to mitochondria and vacuole membranes, the actin cytoskeleton and nuclear chromatin.
Journal ArticleDOI
Butanol production by Clostridium beijerinckii ATCC 55025 from wheat bran
TL;DR: It is suggested that wheat bran can be a potential renewable resource for ABE fermentation, and can utilize hexose and pentose simultaneously in the hydrolysate to produce ABE.
Journal ArticleDOI
Key technologies for the industrial production of fumaric acid by fermentation
TL;DR: The key problems of the industrial production of microbial fumaric acid are reviewed, and various strategies, including strain improvement, morphology control, substrate choice, fermentation process and separation process, and their economical possibilities for industrial processes are discussed.
Journal ArticleDOI
Two-stage utilization of corn straw by Rhizopus oryzae for fumaric acid production.
TL;DR: Under the optimal condition using this two-stage corn straw utilization strategy, the fumaric acid production, was up to 27.79 g/l, with the yield of 0.35 g/g, productivity of 1.33 g/L/h.
Journal ArticleDOI
Comparison of fumaric acid production by Rhizopus oryzae using different neutralizing agents.
TL;DR: The NaHCO3 alternative has advantages of cell reuse and simple downstream processing because of the high solubility of sodium fumarate, and these advantages may offset the disadvantages of using NaH CO3 as the neutralizing agent, and the overall fumaric acid weight yield and volumetric productivity will increase.