Journal ArticleDOI
Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
Zhixiong Xue,Pamela L. Sharpe,Seung-Pyo Hong,Narendra S. Yadav,Dongming Xie,David R. Short,Howard Glenn Damude,Ross A. Rupert,John E. Seip,Jamie Wang,Dana M. Walters Pollak,Michael W. Bostick,Melissa D. Bosak,Daniel Joseph Macool,Dieter Hollerbach,Hongxiang Zhang,Dennis M Arcilla,Sidney A Bledsoe,Kevin Michael Croker,Elizabeth Forrester Mccord,Bjorn D. Tyreus,Ethel N. Jackson,Quinn Qun Zhu +22 more
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TLDR
Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products and provides a sustainable source of EPA.Abstract:
Metabolic engineering of the oleaginous yeast Yarrowia lipolytica greatly enhances yields of omega-3 eicosapentaenoic acid.read more
Citations
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Journal ArticleDOI
Enhanced lipid accumulation in the yeast Yarrowia lipolytica by over-expression of ATP:citrate lyase from Mus musculus.
TL;DR: It is suggested that ACL is an important acetyl-CoA producer and plays a vital role in lipid accumulation in oleaginous yeast Y. lipolytica and had no significant effect on fatty acid profile of the yeast.
Journal ArticleDOI
Metabolic engineering of microbial cell factories for production of nutraceuticals
Shuo-Fu Yuan,Hal S. Alper +1 more
TL;DR: This review covers current strategies of metabolic engineering employed for the production of a few key nutraceuticals with selecting polyunsaturated fatty acids, polyphenolic compounds, carotenoids and non-proteinogenic amino acids as exemplary molecules.
Journal ArticleDOI
Future availability of raw materials for salmon feeds and supply chain implications: the case of Scottish farmed salmon
TL;DR: It is concluded that Scottish salmon farming is a successful example of sustainable feed development and the industry can be confident that the changing raw material base will support continuing production of high quality, healthy farmed salmon, but the long-term security of supply of omega-3 LC-PUFA remains an issue.
Journal ArticleDOI
Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations.
TL;DR: This article aims to provide basic and actualized knowledge on Y. lipolytica engineering technology, with a particular emphasis on promoter tuning, metabolic pathways assembly, and genome editing technologies.
Journal ArticleDOI
Alternative Substrate Metabolism in Yarrowia lipolytica .
TL;DR: A comprehensive understanding of the current state of knowledge for alternative substrate utilization is provided, potential pathways identified through homology in the absence of prior characterization are suggested, and critical questions and challenges for engineering Y. lipolytica for better alternative substrates are described.
References
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Book ChapterDOI
Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
TL;DR: Important insights into the mechanisms of inflammatory responses, pain, and fever have been gleaned from the current understanding of eicosanoid biology.
Journal ArticleDOI
Effects of eicosapentaenoic acid on major coronary events in hypercholesterolaemic patients (JELIS): a randomised open-label, blinded endpoint analysis
Mitsuhiro Yokoyama,Hideki Origasa,Masunori Matsuzaki,Yuji Matsuzawa,Yasushi Saito,Yuichi Ishikawa,Shinichi Oikawa,Jun Sasaki,Hitoshi Hishida,Hiroshige Itakura,Toru Kita,Akira Kitabatake,Noriaki Nakaya,Toshiie Sakata,Kazuyuki Shimada,Kunio Shirato +15 more
TL;DR: Testing the hypothesis that long-term use of eicosapentaenoic acid (EPA) is effective for prevention of major coronary events in hypercholesterolaemic patients in Japan who consume a large amount of fish found it to be a promising treatment.
Journal ArticleDOI
Genome evolution in yeasts
Bernard Dujon,David James Sherman,Gilles Fischer,Pascal Durrens,Serge Casaregola,Ingrid Lafontaine,Jacky de Montigny,Christian Marck,Cécile Neuvéglise,Emmanuel Talla,Nicolas Goffard,Lionel Frangeul,Michel Aigle,Véronique Anthouard,Anna Babour,Valérie Barbe,Stéphanie Barnay,Sylvie Blanchin,Jean-Marie Beckerich,Emmanuelle Beyne,Claudine Bleykasten,Anita Boisramé,Jeanne Boyer,Laurence Cattolico,Fabrice Confanioleri,Antoine de Daruvar,Laurence Despons,Emmanuelle Fabre,Cécile Fairhead,Hélène Ferry-Dumazet,Alexis Groppi,Florence Hantraye,Christophe Hennequin,Nicolas Jauniaux,Philippe Joyet,Rym Kachouri,Alix Kerrest,Romain Koszul,Marc Lemaire,Isabelle Lesur,Laurence Ma,Héloïse Muller,Jean-Marc Nicaud,Macha Nikolski,Sophie Oztas,Odile Ozier-Kalogeropoulos,Stefan Pellenz,Serge Potier,Guy-Franck Richard,Marie-Laure Straub,Audrey Suleau,Dominique Swennen,Fredj Tekaia,Micheline Wésolowski-Louvel,Eric Westhof,Bénédicte Wirth,Maria Zeniou-Meyer,Ivan Zivanovic,Monique Bolotin-Fukuhara,Agnès Thierry,Christiane Bouchier,Bernard Caudron,Claude Scarpelli,Claude Gaillardin,Jean Weissenbach,Patrick Wincker,Jean-Luc Souciet +66 more
TL;DR: Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
Journal ArticleDOI
Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and Eukaryotes
James G. Metz,Paul G. Roessler,Daniel Facciotti,Charlene Levering,Franziska Dittrich,Michael Lassner,Ray Valentine,Kathryn D. Lardizabal,Frédéric Domergue,Akiko Yamada,Kazunaga Yazawa,Vic C. Knauf,John Browse +12 more
TL;DR: In this article, pathways for PUFA synthesis are described that do not require desaturation and elongation of saturated fatty acids and are catalyzed by polyketide synthases (PKSs).