Journal ArticleDOI
Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
Zhixiong Xue,Pamela L. Sharpe,Seung-Pyo Hong,Narendra S. Yadav,Dongming Xie,David R. Short,Howard Glenn Damude,Ross A. Rupert,John E. Seip,Jamie Wang,Dana M. Walters Pollak,Michael W. Bostick,Melissa D. Bosak,Daniel Joseph Macool,Dieter Hollerbach,Hongxiang Zhang,Dennis M Arcilla,Sidney A Bledsoe,Kevin Michael Croker,Elizabeth Forrester Mccord,Bjorn D. Tyreus,Ethel N. Jackson,Quinn Qun Zhu +22 more
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TLDR
Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products and provides a sustainable source of EPA.Abstract:
Metabolic engineering of the oleaginous yeast Yarrowia lipolytica greatly enhances yields of omega-3 eicosapentaenoic acid.read more
Citations
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Journal ArticleDOI
acCRISPR: an activity-correction method for improving the accuracy of CRISPR screens
TL;DR: In this paper , an end-to-end pipeline is developed to identify essential genes in pooled CRISPR screens using sgRNA read counts obtained from next-generation sequencing. But, the pipeline is limited to a small subset of genes.
Journal ArticleDOI
Chromatographic Techniques to Separate and Identify Bioactive Compounds in Microalgae
TL;DR: The findings of this review paper illustrated that chromatographic methods coupled to different types of detectors have been used as a crucial part of research on microalgal polyphenols, Omega-3 Polyunsaturated Fatty Acids (ω-3 PUFAs), and pigments production through identification, measurement, sample preparation, and purification practices.
Book ChapterDOI
Multi-Enzymatic Cascades In Vivo
Lydia Suchy,Florian Rudroff +1 more
TL;DR: In this paper, pros and cons of enzymatic cascades in living cells are described and the current status of the design and the application of them are highlighted, taking Nature as a model, and application of cascade reactions in organic synthesis offers several advantages over the classical step-by-step approach.
Journal ArticleDOI
Multi-Omics Reveals the Effects of Cannabidiol on Gut Microbiota and Metabolic Phenotypes.
TL;DR: In this article , the effect of CBD on the gut microbiota and metabolic phenotype of mice was evaluated using 16S ribosomal RNA (rRNA) gene sequencing and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry-based metabolomics.
Posted ContentDOI
Improving xylose consumption in Rhodotorula toruloides through heterologous expression of xylose reductase and xylulokinase
Paola Monteiro de Oliveira,Marina Julio Pinheiro,Juliano Sabedotti De Biaggi,Eliise Tammekivi,Koit Herodes,Nemailla Bonturi,Petri-Jaan Lahtvee +6 more
TL;DR: In this paper , the xylose consumption pathway in Rhodotorula toruloides was investigated and it was shown that xylosose reductase and xylulokinases showed more significant improvements in terms of xyloses consumption rate compared to the single overexpression of Xylos reductases.
References
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Book ChapterDOI
Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
TL;DR: Important insights into the mechanisms of inflammatory responses, pain, and fever have been gleaned from the current understanding of eicosanoid biology.
Journal ArticleDOI
Effects of eicosapentaenoic acid on major coronary events in hypercholesterolaemic patients (JELIS): a randomised open-label, blinded endpoint analysis
Mitsuhiro Yokoyama,Hideki Origasa,Masunori Matsuzaki,Yuji Matsuzawa,Yasushi Saito,Yuichi Ishikawa,Shinichi Oikawa,Jun Sasaki,Hitoshi Hishida,Hiroshige Itakura,Toru Kita,Akira Kitabatake,Noriaki Nakaya,Toshiie Sakata,Kazuyuki Shimada,Kunio Shirato +15 more
TL;DR: Testing the hypothesis that long-term use of eicosapentaenoic acid (EPA) is effective for prevention of major coronary events in hypercholesterolaemic patients in Japan who consume a large amount of fish found it to be a promising treatment.
Journal ArticleDOI
Genome evolution in yeasts
Bernard Dujon,David James Sherman,Gilles Fischer,Pascal Durrens,Serge Casaregola,Ingrid Lafontaine,Jacky de Montigny,Christian Marck,Cécile Neuvéglise,Emmanuel Talla,Nicolas Goffard,Lionel Frangeul,Michel Aigle,Véronique Anthouard,Anna Babour,Valérie Barbe,Stéphanie Barnay,Sylvie Blanchin,Jean-Marie Beckerich,Emmanuelle Beyne,Claudine Bleykasten,Anita Boisramé,Jeanne Boyer,Laurence Cattolico,Fabrice Confanioleri,Antoine de Daruvar,Laurence Despons,Emmanuelle Fabre,Cécile Fairhead,Hélène Ferry-Dumazet,Alexis Groppi,Florence Hantraye,Christophe Hennequin,Nicolas Jauniaux,Philippe Joyet,Rym Kachouri,Alix Kerrest,Romain Koszul,Marc Lemaire,Isabelle Lesur,Laurence Ma,Héloïse Muller,Jean-Marc Nicaud,Macha Nikolski,Sophie Oztas,Odile Ozier-Kalogeropoulos,Stefan Pellenz,Serge Potier,Guy-Franck Richard,Marie-Laure Straub,Audrey Suleau,Dominique Swennen,Fredj Tekaia,Micheline Wésolowski-Louvel,Eric Westhof,Bénédicte Wirth,Maria Zeniou-Meyer,Ivan Zivanovic,Monique Bolotin-Fukuhara,Agnès Thierry,Christiane Bouchier,Bernard Caudron,Claude Scarpelli,Claude Gaillardin,Jean Weissenbach,Patrick Wincker,Jean-Luc Souciet +66 more
TL;DR: Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
Journal ArticleDOI
Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and Eukaryotes
James G. Metz,Paul G. Roessler,Daniel Facciotti,Charlene Levering,Franziska Dittrich,Michael Lassner,Ray Valentine,Kathryn D. Lardizabal,Frédéric Domergue,Akiko Yamada,Kazunaga Yazawa,Vic C. Knauf,John Browse +12 more
TL;DR: In this article, pathways for PUFA synthesis are described that do not require desaturation and elongation of saturated fatty acids and are catalyzed by polyketide synthases (PKSs).