Journal ArticleDOI
Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
Zhixiong Xue,Pamela L. Sharpe,Seung-Pyo Hong,Narendra S. Yadav,Dongming Xie,David R. Short,Howard Glenn Damude,Ross A. Rupert,John E. Seip,Jamie Wang,Dana M. Walters Pollak,Michael W. Bostick,Melissa D. Bosak,Daniel Joseph Macool,Dieter Hollerbach,Hongxiang Zhang,Dennis M Arcilla,Sidney A Bledsoe,Kevin Michael Croker,Elizabeth Forrester Mccord,Bjorn D. Tyreus,Ethel N. Jackson,Quinn Qun Zhu +22 more
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TLDR
Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products and provides a sustainable source of EPA.Abstract:
Metabolic engineering of the oleaginous yeast Yarrowia lipolytica greatly enhances yields of omega-3 eicosapentaenoic acid.read more
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Journal ArticleDOI
High contents of very long-chain polyunsaturated fatty acids in different moss species
TL;DR: High amounts of very long-chain (vl) PUFAs such as arachidonic acid (AA) in seven moss species are reported on, showing that vlPUFAs are highly abundant metabolites in mosses.
Journal ArticleDOI
Development and physiological characterization of cellobiose‐consuming Yarrowia lipolytica
Stephan Lane,Stephan Lane,Shuyan Zhang,Shuyan Zhang,Na Wei,Christopher V. Rao,Christopher V. Rao,Yong Su Jin,Yong Su Jin +8 more
TL;DR: This study created a Y. lipolytica strain capable of utilizing cellobiose as a sole carbon source by using endogenous promoters to express the cellodextrin transporter cdt‐1 and intracellular β‐glucosidase gh1‐1 from Neurospora crassa and applied it to produce citric acid from bioconversion of crystalline cellulose through simultaneous saccharification and fermentation (SSF).
Journal ArticleDOI
Eicosapentaenoic acid (EPA) production by an oleaginous fungus Mortierella alpina expressing heterologous the Δ17‐desaturase gene under ordinary temperature
Tomoyo Okuda,Akinori Ando,Hiroaki Negoro,Tatsuya Muratsubaki,Hiroshi Kikukawa,Takaiku Sakamoto,Eiji Sakuradani,Sakayu Shimizu,Jun Ogawa +8 more
TL;DR: This study investigates EPA production at an ordinary temperature (28°C) by expressing the Saprolegnia diclina Δ17 desaturase gene (sdd17m) in M. alpina, and provides a platform technology for the industrial production of EPA using M.Alpina as a promising source for EPA.
Journal ArticleDOI
The future of genetic engineering to provide essential dietary nutrients and improve growth performance in aquaculture: Advantages and challenges
Journal ArticleDOI
Microbial conversion of xylose into useful bioproducts
TL;DR: D-xylose may provide a better feedstock for the production of bioproducts other than ethanol, because the relevant pathways are not subject to glucose-dependent repression.
References
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Book ChapterDOI
Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
TL;DR: Important insights into the mechanisms of inflammatory responses, pain, and fever have been gleaned from the current understanding of eicosanoid biology.
Journal ArticleDOI
Effects of eicosapentaenoic acid on major coronary events in hypercholesterolaemic patients (JELIS): a randomised open-label, blinded endpoint analysis
Mitsuhiro Yokoyama,Hideki Origasa,Masunori Matsuzaki,Yuji Matsuzawa,Yasushi Saito,Yuichi Ishikawa,Shinichi Oikawa,Jun Sasaki,Hitoshi Hishida,Hiroshige Itakura,Toru Kita,Akira Kitabatake,Noriaki Nakaya,Toshiie Sakata,Kazuyuki Shimada,Kunio Shirato +15 more
TL;DR: Testing the hypothesis that long-term use of eicosapentaenoic acid (EPA) is effective for prevention of major coronary events in hypercholesterolaemic patients in Japan who consume a large amount of fish found it to be a promising treatment.
Journal ArticleDOI
Genome evolution in yeasts
Bernard Dujon,David James Sherman,Gilles Fischer,Pascal Durrens,Serge Casaregola,Ingrid Lafontaine,Jacky de Montigny,Christian Marck,Cécile Neuvéglise,Emmanuel Talla,Nicolas Goffard,Lionel Frangeul,Michel Aigle,Véronique Anthouard,Anna Babour,Valérie Barbe,Stéphanie Barnay,Sylvie Blanchin,Jean-Marie Beckerich,Emmanuelle Beyne,Claudine Bleykasten,Anita Boisramé,Jeanne Boyer,Laurence Cattolico,Fabrice Confanioleri,Antoine de Daruvar,Laurence Despons,Emmanuelle Fabre,Cécile Fairhead,Hélène Ferry-Dumazet,Alexis Groppi,Florence Hantraye,Christophe Hennequin,Nicolas Jauniaux,Philippe Joyet,Rym Kachouri,Alix Kerrest,Romain Koszul,Marc Lemaire,Isabelle Lesur,Laurence Ma,Héloïse Muller,Jean-Marc Nicaud,Macha Nikolski,Sophie Oztas,Odile Ozier-Kalogeropoulos,Stefan Pellenz,Serge Potier,Guy-Franck Richard,Marie-Laure Straub,Audrey Suleau,Dominique Swennen,Fredj Tekaia,Micheline Wésolowski-Louvel,Eric Westhof,Bénédicte Wirth,Maria Zeniou-Meyer,Ivan Zivanovic,Monique Bolotin-Fukuhara,Agnès Thierry,Christiane Bouchier,Bernard Caudron,Claude Scarpelli,Claude Gaillardin,Jean Weissenbach,Patrick Wincker,Jean-Luc Souciet +66 more
TL;DR: Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
Journal ArticleDOI
Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and Eukaryotes
James G. Metz,Paul G. Roessler,Daniel Facciotti,Charlene Levering,Franziska Dittrich,Michael Lassner,Ray Valentine,Kathryn D. Lardizabal,Frédéric Domergue,Akiko Yamada,Kazunaga Yazawa,Vic C. Knauf,John Browse +12 more
TL;DR: In this article, pathways for PUFA synthesis are described that do not require desaturation and elongation of saturated fatty acids and are catalyzed by polyketide synthases (PKSs).