Journal ArticleDOI
Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
Zhixiong Xue,Pamela L. Sharpe,Seung-Pyo Hong,Narendra S. Yadav,Dongming Xie,David R. Short,Howard Glenn Damude,Ross A. Rupert,John E. Seip,Jamie Wang,Dana M. Walters Pollak,Michael W. Bostick,Melissa D. Bosak,Daniel Joseph Macool,Dieter Hollerbach,Hongxiang Zhang,Dennis M Arcilla,Sidney A Bledsoe,Kevin Michael Croker,Elizabeth Forrester Mccord,Bjorn D. Tyreus,Ethel N. Jackson,Quinn Qun Zhu +22 more
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TLDR
Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products and provides a sustainable source of EPA.Abstract:
Metabolic engineering of the oleaginous yeast Yarrowia lipolytica greatly enhances yields of omega-3 eicosapentaenoic acid.read more
Citations
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Journal ArticleDOI
Metabolic engineering of oleaginous yeasts to enhance single cell oil production
Journal ArticleDOI
Progress of metabolic engineering for the production of eicosapentaenoic acid.
Yu-Lei Jia,Shan-Shan Geng,Fei Du,Ying-Shuang Xu,Ling-Ru Wang,Xiao-Man Sun,Qingzhuo Wang,Qi Li +7 more
TL;DR: In this article, a review summarizes recent research on different microorganisms capable of producing EPA, such as microalgae, bacteria, and fungi, and introduces the different EPA biosynthesis pathways.
Journal ArticleDOI
Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway
Wang Ma,Mengzhen Liu,Zi-Xu Zhang,Ying-Shuang Xu,Peng-Wei Huang,Dong-Sheng Guo,Xiao-Man Sun,He Huang +7 more
TL;DR: In this article , a methionine synthase-like (MetE-like) complex was proposed for co-production of eicosapentaenoic acid (EPA) and DHA.
Book ChapterDOI
Microbial bioprocessing of health promoting food supplements
TL;DR: This chapter is focused on the synthesis of biofunctional food supplements by conventional and metabolic engineered microorganisms, safety, and regulatory issues.
Journal ArticleDOI
Production of human milk fat substitute by engineered strains of Yarrowia lipolytica
TL;DR: In this paper , an oleaginous yeast can be engineered to produce a human milk fat substitute (β-palmitate), that could be used as an ingredient in infant formulas.
References
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Book ChapterDOI
Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
TL;DR: Important insights into the mechanisms of inflammatory responses, pain, and fever have been gleaned from the current understanding of eicosanoid biology.
Journal ArticleDOI
Effects of eicosapentaenoic acid on major coronary events in hypercholesterolaemic patients (JELIS): a randomised open-label, blinded endpoint analysis
Mitsuhiro Yokoyama,Hideki Origasa,Masunori Matsuzaki,Yuji Matsuzawa,Yasushi Saito,Yuichi Ishikawa,Shinichi Oikawa,Jun Sasaki,Hitoshi Hishida,Hiroshige Itakura,Toru Kita,Akira Kitabatake,Noriaki Nakaya,Toshiie Sakata,Kazuyuki Shimada,Kunio Shirato +15 more
TL;DR: Testing the hypothesis that long-term use of eicosapentaenoic acid (EPA) is effective for prevention of major coronary events in hypercholesterolaemic patients in Japan who consume a large amount of fish found it to be a promising treatment.
Journal ArticleDOI
Genome evolution in yeasts
Bernard Dujon,David James Sherman,Gilles Fischer,Pascal Durrens,Serge Casaregola,Ingrid Lafontaine,Jacky de Montigny,Christian Marck,Cécile Neuvéglise,Emmanuel Talla,Nicolas Goffard,Lionel Frangeul,Michel Aigle,Véronique Anthouard,Anna Babour,Valérie Barbe,Stéphanie Barnay,Sylvie Blanchin,Jean-Marie Beckerich,Emmanuelle Beyne,Claudine Bleykasten,Anita Boisramé,Jeanne Boyer,Laurence Cattolico,Fabrice Confanioleri,Antoine de Daruvar,Laurence Despons,Emmanuelle Fabre,Cécile Fairhead,Hélène Ferry-Dumazet,Alexis Groppi,Florence Hantraye,Christophe Hennequin,Nicolas Jauniaux,Philippe Joyet,Rym Kachouri,Alix Kerrest,Romain Koszul,Marc Lemaire,Isabelle Lesur,Laurence Ma,Héloïse Muller,Jean-Marc Nicaud,Macha Nikolski,Sophie Oztas,Odile Ozier-Kalogeropoulos,Stefan Pellenz,Serge Potier,Guy-Franck Richard,Marie-Laure Straub,Audrey Suleau,Dominique Swennen,Fredj Tekaia,Micheline Wésolowski-Louvel,Eric Westhof,Bénédicte Wirth,Maria Zeniou-Meyer,Ivan Zivanovic,Monique Bolotin-Fukuhara,Agnès Thierry,Christiane Bouchier,Bernard Caudron,Claude Scarpelli,Claude Gaillardin,Jean Weissenbach,Patrick Wincker,Jean-Luc Souciet +66 more
TL;DR: Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
Journal ArticleDOI
Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and Eukaryotes
James G. Metz,Paul G. Roessler,Daniel Facciotti,Charlene Levering,Franziska Dittrich,Michael Lassner,Ray Valentine,Kathryn D. Lardizabal,Frédéric Domergue,Akiko Yamada,Kazunaga Yazawa,Vic C. Knauf,John Browse +12 more
TL;DR: In this article, pathways for PUFA synthesis are described that do not require desaturation and elongation of saturated fatty acids and are catalyzed by polyketide synthases (PKSs).