Journal ArticleDOI
Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica
Zhixiong Xue,Pamela L. Sharpe,Seung-Pyo Hong,Narendra S. Yadav,Dongming Xie,David R. Short,Howard Glenn Damude,Ross A. Rupert,John E. Seip,Jamie Wang,Dana M. Walters Pollak,Michael W. Bostick,Melissa D. Bosak,Daniel Joseph Macool,Dieter Hollerbach,Hongxiang Zhang,Dennis M Arcilla,Sidney A Bledsoe,Kevin Michael Croker,Elizabeth Forrester Mccord,Bjorn D. Tyreus,Ethel N. Jackson,Quinn Qun Zhu +22 more
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TLDR
Inactivation of the peroxisome biogenesis gene PEX10 was crucial in obtaining high EPA yields and may increase the yields of other commercially desirable lipid-related products and provides a sustainable source of EPA.Abstract:
Metabolic engineering of the oleaginous yeast Yarrowia lipolytica greatly enhances yields of omega-3 eicosapentaenoic acid.read more
Citations
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Journal ArticleDOI
Potential of Mortierellaceae for polyunsaturated fatty acids production: mini review
Journal ArticleDOI
Combination of a Push–Pull–Block Strategy with a Heterologous Xylose Assimilation Pathway toward Lipid Overproduction from Lignocellulose in <i>Yarrowia lipolytica</i>
TL;DR: In this paper , an engineered Yarrowia lipolytica strain that can efficiently produce lipids from glucose and xylose was constructed. And the final evolved strain could accumulate 53.64% of the dry cell weight (DCW).
Journal ArticleDOI
Antibiotic-induced gut microbiota dysbiosis altered host metabolism.
TL;DR: In this paper , the authors found that taking antibiotics reduced the diversity and richness of gut microbiota and affected the composition of the microbiome, which in turn altered the metabolic profiles of plasma and fecal samples, and the most significant pathways related to the gut dysbiosis induced by antibiotics including purine, pentose and glucuronate metabolism, histidine, ascorbate and alternate, lysine degradation, and fatty acid biosynthesis.
Posted ContentDOI
Proteomes Reveal Metabolic Capabilities of Yarrowia lipolytica for Biological Upcycling of Polyethylene into High-Value Chemicals
Caleb Walker,Christopher Cotter,Ikenna Okekeogbu,Seung-Kon Ryu,Bamin Khomami,Richard J. Giannone,Siris Odin Laursen,Cong T. Trinh +7 more
TL;DR: In this article , an oleaginous yeast Yarrowia lipolytica undergoing short-term adaptation to depolymerized plastic (DP) oil robustly utilized a wide range of hydrocarbons for cell growth and production of citric acid and neutral lipids.
Book ChapterDOI
Western Blotting of Membrane-Bound Proteins in Yarrowia lipolytica.
Adithya Ramesh,Ian Wheeldon +1 more
TL;DR: In this paper, the authors present a method for easy detection and quantification of integral membrane proteins in the industrially important oleaginous yeast Yarrowia lipolytica.
References
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Book ChapterDOI
Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
TL;DR: Important insights into the mechanisms of inflammatory responses, pain, and fever have been gleaned from the current understanding of eicosanoid biology.
Journal ArticleDOI
Effects of eicosapentaenoic acid on major coronary events in hypercholesterolaemic patients (JELIS): a randomised open-label, blinded endpoint analysis
Mitsuhiro Yokoyama,Hideki Origasa,Masunori Matsuzaki,Yuji Matsuzawa,Yasushi Saito,Yuichi Ishikawa,Shinichi Oikawa,Jun Sasaki,Hitoshi Hishida,Hiroshige Itakura,Toru Kita,Akira Kitabatake,Noriaki Nakaya,Toshiie Sakata,Kazuyuki Shimada,Kunio Shirato +15 more
TL;DR: Testing the hypothesis that long-term use of eicosapentaenoic acid (EPA) is effective for prevention of major coronary events in hypercholesterolaemic patients in Japan who consume a large amount of fish found it to be a promising treatment.
Journal ArticleDOI
Genome evolution in yeasts
Bernard Dujon,David James Sherman,Gilles Fischer,Pascal Durrens,Serge Casaregola,Ingrid Lafontaine,Jacky de Montigny,Christian Marck,Cécile Neuvéglise,Emmanuel Talla,Nicolas Goffard,Lionel Frangeul,Michel Aigle,Véronique Anthouard,Anna Babour,Valérie Barbe,Stéphanie Barnay,Sylvie Blanchin,Jean-Marie Beckerich,Emmanuelle Beyne,Claudine Bleykasten,Anita Boisramé,Jeanne Boyer,Laurence Cattolico,Fabrice Confanioleri,Antoine de Daruvar,Laurence Despons,Emmanuelle Fabre,Cécile Fairhead,Hélène Ferry-Dumazet,Alexis Groppi,Florence Hantraye,Christophe Hennequin,Nicolas Jauniaux,Philippe Joyet,Rym Kachouri,Alix Kerrest,Romain Koszul,Marc Lemaire,Isabelle Lesur,Laurence Ma,Héloïse Muller,Jean-Marc Nicaud,Macha Nikolski,Sophie Oztas,Odile Ozier-Kalogeropoulos,Stefan Pellenz,Serge Potier,Guy-Franck Richard,Marie-Laure Straub,Audrey Suleau,Dominique Swennen,Fredj Tekaia,Micheline Wésolowski-Louvel,Eric Westhof,Bénédicte Wirth,Maria Zeniou-Meyer,Ivan Zivanovic,Monique Bolotin-Fukuhara,Agnès Thierry,Christiane Bouchier,Bernard Caudron,Claude Scarpelli,Claude Gaillardin,Jean Weissenbach,Patrick Wincker,Jean-Luc Souciet +66 more
TL;DR: Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
Journal ArticleDOI
Production of Polyunsaturated Fatty Acids by Polyketide Synthases in Both Prokaryotes and Eukaryotes
James G. Metz,Paul G. Roessler,Daniel Facciotti,Charlene Levering,Franziska Dittrich,Michael Lassner,Ray Valentine,Kathryn D. Lardizabal,Frédéric Domergue,Akiko Yamada,Kazunaga Yazawa,Vic C. Knauf,John Browse +12 more
TL;DR: In this article, pathways for PUFA synthesis are described that do not require desaturation and elongation of saturated fatty acids and are catalyzed by polyketide synthases (PKSs).