Journal ArticleDOI
Proteomics of human aqueous humor.
Krishna R Murthy,Pavithra Rajagopalan,Sneha M. Pinto,Jayshree Advani,Praveen R Murthy,Renu Goel,Yashwanth Subbannayya,Lavanya Balakrishnan,Mahashweta Dash,Abhijith K. Anil,Srikanth S. Manda,Raja Sekhar Nirujogi,Dhanashree S. Kelkar,Gajanan Sathe,Gourav Dey,Aditi Chatterjee,Harsha Gowda,Shukti Chakravarti,Subramanian Shankar,Nandini A. Sahasrabuddhe,Bipin G. Nair,B. L. Somani,T. S. Keshava Prasad,Akhilesh Pandey +23 more
TLDR
This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.Abstract:
The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier. Though this ocular fluid has been extensively studied, its role in ocular physiology is still not completely understood. In this study, aqueous humor samples were collected from 250 patients undergoing cataract surgery, subjected to multiple fractionation strategies and analyzed on a Fourier transform LTQ-Orbitrap Velos mass spectrometer. In all, we identified 763 proteins, of which 386 have been identified for the first time in this study. Sorbitol dehydrogenase (SORD), filensin (BFSP1), and phakinin (BFSP2) are some of the proteins that have not been previously reported in the aqueous humor. Gene Ontology analysis revealed 35% of the identified proteins to be extracellular, with a majority of them involved in cell communication and signal transduction. This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.read more
Citations
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Pigment epithelium derived factor:a potent inhibitor of angiogenesis
TL;DR: Pigment epithelium-derived factor may prove to be a useful therapeutic for neuronal damage and inhibiting angiogenesis may be particularly effective, as it is taken as one of the most effective antiangiogenic factors.
Journal ArticleDOI
Roles of exosomes in the normal and diseased eye.
TL;DR: Current knowledge of exosome function in the visual system is reviewed in the context of larger bodies of data from other fields, in both health and disease, to identify areas of opportunity for future research studies.
Journal ArticleDOI
Review of Biomarkers in Ocular Matrices: Challenges and Opportunities.
TL;DR: This review describes the usage of biomarkers with respect to four commonly sampled ocular matrices in clinic: tears, conjunctiva, aqueous humor and vitreous.
Journal ArticleDOI
The Human Eye Proteome Project: Updates on an Emerging Proteome.
TL;DR: This updated catalogue sheds light on the molecular makeup of previously undescribed proteomes within the human eye, including optic nerve, sclera, iris, and ciliary body, while adding additional proteins to previously characterized proteomes such as aqueous humor, lens, vitreous, retina, and retinal pigment epithelium/choroid.
Journal ArticleDOI
Human aqueous humor proteome in cataract, glaucoma, and pseudoexfoliation syndrome
Anna A. Kliuchnikova,Nadezhda I Samokhina,Irina Y. Ilina,Dmitry S. Karpov,Mikhail A. Pyatnitskiy,Ksenia G. Kuznetsova,Ilya Yu. Toropygin,Sergey A. Kochergin,Igor’ B Alekseev,Victor G. Zgoda,Alexander I. Archakov,Sergei A. Moshkovskii +11 more
TL;DR: Twenty‐nine human aqueous humor samples from patients with eye diseases such as cataract and glaucoma with and without pseudoexfoliation syndrome were characterized by LC–high resolution MS analysis, finding decrease in the level of apolipoprotein D as a marker of the pseudoexporiation syndrome.
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