Journal ArticleDOI
Purification and analysis of an extremely halophilic β-galactosidase from Haloferax alicantei
Melissa L. Holmes,Robert K. Scopes,Robert L. Moritz,Richard J. Simpson,Christoph Englert,Felicitas Pfeifer,Michael L. Dyall-Smith +6 more
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TLDR
As a first step in the development of a reporter system for gene expression in halophilic archaea, a beta-galactosidase was purified 140-fold from Haloferax alicantei (previously phenon K, strain Aa2.2).About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1997-02-08. It has received 110 citations till now. The article focuses on the topics: Fucosidase activity & Haloferax.read more
Citations
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Potential of halotolerant and halophilic microorganisms for biotechnology.
Rosa Margesin,Franz Schinner +1 more
TL;DR: Halotolerant microorganisms play an essential role in food biotechnology for the production of fermented food and food supplements and the degradation or transformation of a range of organic pollutants and theproduction of alternative energy are other fields of applications of these groups of extremophiles.
Journal ArticleDOI
Halophilic adaptation of enzymes.
TL;DR: It is described how thermodynamic observations, such as parameters pertaining to solvent–protein interactions or enzyme-unfolding kinetics, depend strongly on solvent composition and reveal the important role played by water and ion binding to halophilic proteins.
Journal ArticleDOI
Enzymes from extremophiles.
TL;DR: Enzymes from thermophilic organisms have found the most practical commercial use to date because of their overall inherent stability, which has led to a greater understanding of stability factors involved in adaptation of these enzymes to their unusual environments.
Journal ArticleDOI
Biocatalysis in organic media using enzymes from extremophiles
TL;DR: This review introduces organic media bioc atalysis before addressing the state of the art of recent fundamental and applied aspects of extremozyme biocatalysis, and enhancing extremo enzyme performance via chemical modification, complexation, immobilization, and protein engineering.
BookDOI
Adaptation to life at high salt concentrations in archaea, bacteria, and eukarya
TL;DR: This paper presents a comparative genomic survey of information transfer systems in two diverse extremely halophilic Archaea, Halobacterium sp.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Classification of Non-alkaliphilic Halobacteria Based on Numerical Taxonomy and Polar Lipid Composition, and Description of Haloarcula gen. nov. and Haloferax gen. nov.
Marina Torreblanca,Francisco Rodriguez-Valera,G. Juez,Antonio Ventosa,Masahiro Kamekura,Morris Kates +5 more
TL;DR: The results show the existence of at least three groups with the taxonomic level of genus Halobacterium, and propose the two new genera Haloarcula and Haloferax for the nomenclatural accomodation of the three groups.
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