Book ChapterDOI
Quantitation of nitrate and nitrite in extracellular fluids
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This article is published in Methods in Enzymology.The article was published on 1996-01-01. It has received 476 citations till now. The article focuses on the topics: Nitrate reductase & Nitrate.read more
Citations
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Journal ArticleDOI
A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite.
TL;DR: A method for simultaneous evaluation of nitrate and nitrite concentrations in a microtiter plate format is developed and S-Nitrosothiols and L-arginine derivatives were found to be potential interfering agents.
Journal ArticleDOI
Antioxidant activity of food constituents: an overview
TL;DR: The most commonly methods used in vitro determination of antioxidant capacity of food constituents are reviewed and presented, and the general chemistry underlying the assays in the present paper was clarified.
Journal ArticleDOI
Methods to detect nitric oxide and its metabolites in biological samples
TL;DR: The methods described in this review are not an exhaustive or comprehensive discussion of all methods available for the detection of NO but rather a description of the most commonly used and practical methods which allow accurate and sensitive quantification of NO products/metabolites in multiple biological matrices under normal physiological conditions.
Journal ArticleDOI
NITRATE REDUCTASE STRUCTURE, FUNCTION AND REGULATION: Bridging the Gap between Biochemistry and Physiology.
TL;DR: A complete three-dimensional dimeric NR structure model was built from structures of sulfite oxidase and cytochrome b reductase, and key active site residues have been investigated.
Journal ArticleDOI
Antioxidants and antioxidant methods: an updated overview
TL;DR: Antioxidants had a growing interest owing to their protective roles in food and pharmaceutical products against oxidative deterioration and in the body and against oxidative stress-mediated pathological processes as discussed by the authors, and many studies evaluating the antioxidant activity of various samples of research interest have been conducted.
References
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Journal ArticleDOI
The L-Arginine-Nitric Oxide Pathway
Salvador Moncada,Annie Higgs +1 more
TL;DR: The discovery that mammalian cells generate nitric oxide, a gas previously considered to be merely an atmospheric pollutant, is providing important information about many biologic processes.
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Nitric oxide as a secretory product of mammalian cells.
TL;DR: How different forms of nitric oxide synthase help confer specificity and diversity on the effects of this remarkable signaling molecule is reviewed.
Journal ArticleDOI
Increased concentrations of nitrite in synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases.
TL;DR: Increased nitric oxide production in rheumatoid arthritis and osteoarthritis is measured indirectly as nitrite in serum and synovial fluid samples together with serum samples from healthy volunteers matched for age and sex to suggest a role forNitric oxide as an inflammatory mediator in r heumatic diseases.
Journal ArticleDOI
Suppression of arthritis by an inhibitor of nitric oxide synthase.
Nancy McCartney-Francis,J B Allen,Diane Mizel,Jorge E. Albina,Qiao-wen Xie,Carl Nathan,Sharon M. Wahl +6 more
TL;DR: It is shown here that NO production is elevated in the inflamed joints of SCW-treated rats and the ability of a NOS inhibitor to modulate the disease is demonstrated.
Journal ArticleDOI
Oxidation of nitrogen oxides by bound dioxygen in hemoproteins.
TL;DR: Details are provided of the stoichiometric transformation through which nitric oxide is converted to nitrate with accompanying oxidation of myoglobin or hemoglobin to the corresponding iron(III) hemoprotein, including an estimate of the rate constant.