Reconstitutions of plasmid partition systems and their mechanisms
Adam C. Brooks,Ling Chin Hwang +1 more
TLDR
The reconstitutions of plasmid partition systems in cell-free reactions are reviewed, and recent work that has begun to challenge long standing models of DNA segregation in bacteria are discussed.About:
This article is published in Plasmid.The article was published on 2017-05-01 and is currently open access. It has received 33 citations till now.read more
Citations
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Going around in circles: virulence plasmids in enteric pathogens
Giulia Pilla,Christoph M. Tang +1 more
TL;DR: Virulence plasmids in Enterobacteriaceae that are important causes of diarrhoea in humans, Shigella spp.
Journal ArticleDOI
Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.
TL;DR: This work focuses on key genetic elements and their encoded proteins present on the F-factor and other typical F-like plasmids belonging to the MOBF12A group of conjugative plasmid, which are present in enterobacterial hosts isolated from clinical as well as environmental samples all over the world.
Journal ArticleDOI
Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes.
TL;DR: How different bacterial species adapt the DNA partitioning ParAB-parS system to meet their specific requirements is highlighted, including ori positioning and anchoring, DNA condensation, and loading of the structural maintenance of chromosome proteins.
Journal ArticleDOI
Genomic Analysis of Mic1 Reveals a Novel Freshwater Long-Tailed Cyanophage
Feng Yang,Hua Jin,Xiao-Qian Wang,Qiong Li,Jun-Tao Zhang,Ning Cui,Yong-Liang Jiang,Yuxing Chen,Qingfa Wu,Cong-Zhao Zhou,Wei-Fang Li +10 more
TL;DR: A long-tailed cyanophage isolated from Lake Chaohu, termed Mic1, is reported, which specifically infects the cyanobacterium Microcystis aeruginosa, and phylogenetic analysis suggested Mic1 and mitochondria share a common evolutionary origin of DNA polymerase γ gene.
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Reconsidering plasmid maintenance factors for computational plasmid design
TL;DR: This work summarizes current knowledge of the plasmid genome organization and the factors that can affect plasmod persistence, with the aim of constructing synthetic plasmids for use in gram-negative bacteria and introduces publicly available resources, plasmide data, and bioinformatics tools that are useful for computational plasmID design.
References
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Spatial regulators for bacterial cell division self-organize into surface waves in vitro.
TL;DR: A reaction-diffusion model of the MinD and MinE dynamics is presented that accounts for the experimental observations and also captures the in vivo oscillations and formed planar surface waves on a flat membrane in vitro.
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A systems study reveals concurrent activation of AMPK and mTOR by amino acids
Piero Dalle Pezze,Stefanie Ruf,Stefanie Ruf,Annika G Sonntag,Miriam Langelaar-Makkinje,Philip Hall,Alexander Martin Heberle,Patricia Razquin Navas,Patricia Razquin Navas,Karen van Eunen,Regine C Tölle,Jennifer J. Schwarz,Heike Wiese,Bettina Warscheid,Jana Deitersen,Björn Stork,Erik Fäßler,Sascha Schäuble,Udo Hahn,Peter Horvatovich,Daryl P. Shanley,Kathrin Thedieck,Kathrin Thedieck +22 more
TL;DR: It is found that aa acutely activate AMPK concurrently with mTOR, and it is shown that AMPK under aa sufficiency acts to sustain autophagy, which may be required to maintain protein homoeostasis and deliver metabolite intermediates for biosynthetic processes.
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A spindle-like apparatus guides bacterial chromosome segregation
Jerod L. Ptacin,Steven F. Lee,Ethan C. Garner,Esteban Toro,Michael Eckart,Luis R. Comolli,W. E. Moerner,Lucy Shapiro +7 more
TL;DR: A bacterial chromosome segregation mechanism that features basic operating principles similar to eukaryotic mitotic machines is elucidated, including a multivalent protein complex at the centromere that stimulates the dynamic disassembly of polymers to move chromosomes into daughter compartments.
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Segregation of molecules at cell division reveals native protein localization
TL;DR: It is found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers.
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Pushing and Pulling in Prokaryotic DNA Segregation
TL;DR: In prokaryotes, DNA can be segregated by three different types of cytoskeletal filaments, and the best-understood type of partitioning (par) locus encodes an actin homolog called ParM, which forms dynamically unstable filaments that push plasmids apart in a process reminiscent of mitosis.