Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
Yuko Kondo-Takuma,Masayuki Mizuno,Yo Tsuda,Yuta Madokoro,Kengo Suzuki,Toyohiro Sato,Hiroshi Takase,Yuto Uchida,Kenichi Adachi,Hideki Hida,Cesario V. Borlongan,Noriyuki Matsukawa +11 more
TLDR
In this article, the authors confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method, suggesting that Ach may be a cholinergic regulator in the septo-hippocampal network.Abstract:
The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP), which induces acetylcholine (Ach) synthesis in the medial septal nuclei of an explant culture system, was purified from the soluble fraction of postnatal rat hippocampus. HCNP is processed from the N-terminal region of a 186-amino acid, 21-kDa HCNP precursor protein, also known as Raf kinase inhibitory protein and phosphatidylethanolamine-binding protein 1. Here, we confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method. The levels of vesicular acetylcholine transporter were also decreased in the hippocampus of these mice in comparison with those in control mice, suggesting there was decreased incorporation of Ach into the synaptic vesicle. These results potently indicate that HCNP may be a cholinergic regulator in the septo-hippocampal network.read more
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Reduction of glutamatergic activity through cholinergic dysfunction in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
Kengo Suzuki,Yoshiaki Ohi,Toyohiro Sato,Yoshitaka Tsuda,Yuta Madokoro,Masayuki Mizuno,Kenichi Adachi,Yuto Uchida,Akira Haji,Kosei Ojika,Noriyuki Matsukawa +10 more
TL;DR: In this paper , the importance of hippocampal cholinergic neuro-stimulating peptide (HCNP) in inducing acetylcholine synthesis in the medial septal nucleus was investigated.
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Reduction of glutamatergic activity through cholinergic dysfunction in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
TL;DR: In this paper , it was shown that HCNP-precursor protein (pp) knockout mice electrophysiologically presented with glutamatergic dysfunction in the hippocampus with age.
Journal ArticleDOI
Direct Enhancement Effect of Hippocampal Cholinergic Neurostimulating Peptide on Cholinergic Activity in the Hippocampus
Yuta Madokoro,Daisuke Kato,Yoshitaka Tsuda,Itsumi Arakawa,Kengo Suzuki,Toyohiro Sato,Masayuki Mizuno,Yuto Uchida,Kosei Ojika,Noriyuki Matsukawa +9 more
TL;DR: In this article , the authors investigated whether hippocampal cholinergic neurostimulating peptide (HCNP) has a rescue function in the cholineergic dysfunction of HCNP-pp conditional knockout (cKO).
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