Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombination.
TLDR
The authors showed that break repair in both noncrossovers and crossover-specific double Holliday junction formation occurs via processes involving branch migration as an integral feature, one that can be separated from repair of the break itself.About:
This article is published in Molecular Cell.The article was published on 2021-10-21 and is currently open access. It has received 19 citations till now. The article focuses on the topics: Strand invasion & Branch migration.read more
Citations
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Recombination between heterologous human acrocentric chromosomes
Andrea Guarracino,Silvia Buonaiuto,Leonardo Gomes de Lima,Tamara A. Potapova,Arang Rhie,Sergey Koren,Boris Rubinstein,Christian Fischer,Jennifer L. Gerton,Adam M. Phillippy,Vincenza Colonna,Erik Garrison +11 more
TL;DR: In the first complete assembly of a human genome, the Telomere-to-Telomere Consortium's CHM13 assembly (T2T-CHM13) provided a model of their homology as mentioned in this paper .
Journal ArticleDOI
Gene conversion: a non-Mendelian process integral to meiotic recombination
TL;DR: Meiosis is a specialised, reductional cell division which generates haploid gametes (reproductive cells) carrying a single chromosome complement from diploid progenitor cells harboring two chromosome sets as mentioned in this paper .
Posted ContentDOI
Turning coldspots into hotspots: targeted recruitment of axis protein Hop1 stimulates meiotic recombination in Saccharomyces cerevisiae
TL;DR: A novel approach that uses a bacterial ParB-parS partition system to recruit axis proteins at high levels to inserts at recombination coldspots where Hop1 and Red1 levels are normally low is developed, raising the intriguing possibility that different recombination pathways operate in different parts of the genome.
Journal ArticleDOI
Diffusion and distal linkages govern interchromosomal dynamics during meiotic prophase
TL;DR: In this article , random diffusion and the placement of a small number of linkages are sufficient to establish the apparent "pairing" of homologs, and colocalization between any two loci is more dynamic than anticipated.
Posted ContentDOI
Diffusion and distal linkages govern interchromosomal dynamics during meiotic prophase
Trent Newman,Bruno Beltran,James McGehee,Daniel Elnatan,Cori K. Cahoon,Michael R. Paddy,Daniel B. Chu,Andrew J. Spakowitz,Sean M. Burgess +8 more
Abstract: The pairing of homologous chromosomes (homologs) in meiosis is essential for distributing the correct numbers of chromosomes into haploid gametes. In budding yeast, pairing depends on the formation of 150-200 Spo11-mediated double-strand breaks (DSBs) that are distributed among 16 homolog pairs, but it is not known if all, or only a subset of these DSBs, contribute to the close juxtaposition of homologs. Having established a system to measure the position of fluorescently tagged chromosomal loci in 3D space over time, we analyzed locus trajectories to determine how frequently, and how long, loci spend colocalized or apart. Continuous imaging revealed highly heterogeneous cell-to-cell behavior of foci, with the majority of cells exhibiting a 99mixed99 phenotype where foci move into and out of proximity, even at late stages of prophase, suggesting that the axial structures of the synaptonemal complex may be more dynamic than anticipated. The observed plateaus of the mean-squared change in distance (MSCD) between foci informed the development of a biophysical model of two diffusing polymers that captures the loss of centromere linkages as cells enter meiosis, nuclear confinement, and the formation of Spo11-dependent linkages. The predicted number of linkages per chromosome in our theoretical model closely approximates the small number (2-4) of estimated synapsis-initiation sites, suggesting that excess DSBs have negligible effects on the overall juxtaposition of homologs. These insights into the dynamic interchromosomal behavior displayed during homolog pairing demonstrate the power of combining time-resolved in vivo analysis with modeling at the granular level.
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