Journal ArticleDOI
Repriming of DNA synthesis at stalled replication forks by human PrimPol
Silvana Mouron,Sara Rodriguez-Acebes,María I. Martínez-Jiménez,Sara García-Gómez,E. Sandra Chocron,Luis Blanco,Juan Méndez +6 more
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TLDR
PrimPol, a new human primase and TLS polymerase, uses its primase activity to mediate uninterrupted fork progression after UV irradiation and to reinitiate DNA synthesis after dNTP depletion, and might become a target for cancer therapy.Abstract:
DNA damage that causes replication forks to stall can be bypassed via translesion synthesis (TLS). New work has identified a bifunctional human primase and TLS polymerase, PrimPol, that reinitiates DNA synthesis beyond the damage site by virtue of its unique primase activity, revealing a novel pathway of DNA-damage tolerance.read more
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The essential kinase ATR: ensuring faithful duplication of a challenging genome
TL;DR: This Review examines how the replication stress response that is controlled by the kinase ataxia telangiectasia and Rad3-related (ATR) senses and resolves threats to DNA integrity so that the DNA remains available to read in all of the authors' cells.
Journal ArticleDOI
Exploiting replicative stress to treat cancer
TL;DR: Current and future approaches to increase replicative stress in cancer therapeutics are outlined, emphasizing the combination of conventional chemotherapy with targeted approaches.
Journal ArticleDOI
Main steps in DNA double-strand break repair: an introduction to homologous recombination and related processes.
TL;DR: In this paper, the main pathways of eukaryotic DNA double-strand break repair with the focus on homologous recombination and its various subpathways are reviewed.
Journal ArticleDOI
Replication stress: getting back on track.
TL;DR: The emerging mechanisms of the replication-stress response in mammalian cells are reviewed and how they may influence the dynamics of the core DNA replication complex is considered.
References
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Journal ArticleDOI
NIH Image to ImageJ: 25 years of image analysis
TL;DR: The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Book
Using Antibodies: A Laboratory Manual
Ed Harlow,David A. Lane +1 more
TL;DR: Epitope Mapping Determining the Structural Requirements Of An Epitope Choosing An EpITope-mapping Method Mapping By Competition Assay Mapping by Expression of Gene Fragments Mapping Using Synthetic Peptides Synthesis Or Purchase Of The Peptide Set Alternate Approaches To Epitopes.
Journal ArticleDOI
Chromatin Association of Human Origin Recognition Complex, Cdc6, and Minichromosome Maintenance Proteins during the Cell Cycle: Assembly of Prereplication Complexes in Late Mitosis
Juan Méndez,Bruce Stillman +1 more
TL;DR: Using centrifugal elutriation of several human cell lines, it is demonstrated that whereas human Orc2 and hMcm proteins are present throughout the cell cycle, hCdc6p levels vary, being very low in early G1 and accumulating until cells enter mitosis, indicating that the mitotic kinase activity inhibits prereplication complex formation in human cells.
Journal ArticleDOI
Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells.
Dean A. Jackson,Ana Pombo +1 more
TL;DR: It is proposed that the coordinated replication of related groups of replicons, that form stable replicon clusters, contributes to the efficient activation and propagation of S phase in mammalian cells.