Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
Xiaoguo Zhang,Xinxin Ding,Richard S. Marshall,Julio Paez-Valencia,Patrick Lacey,Richard D. Vierstra,Marisa S. Otegui +6 more
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TLDR
It is proposed that maize Rtn1 and Rtn2 act as receptors for autophagy-mediated ER turnover, and thus are critical for ER homeostasis and suppression of ER stress.Abstract:
Reticulon (Rtn) proteins shape tubular domains of the endoplasmic reticulum (ER), and in some cases are autophagy receptors for selective ER turnover. We have found that maize Rtn1 and Rtn2 control ER homeostasis and autophagic flux in endosperm aleurone cells, where the ER accumulates lipid droplets and synthesizes storage protein accretions metabolized during germination. Maize Rtn1 and Rtn2 are expressed in the endosperm, localize to the ER, and re-model ER architecture in a dose-dependent manner. Rtn1 and Rtn2 interact with Atg8a using four Atg8-interacting motifs (AIMs) located at the C-terminus, cytoplasmic loop, and within the transmembrane segments. Binding between Rtn2 and Atg8 is elevated upon ER stress. Maize rtn2 mutants display increased autophagy and up-regulation of an ER stress-responsive chaperone. We propose that maize Rtn1 and Rtn2 act as receptors for autophagy-mediated ER turnover, and thus are critical for ER homeostasis and suppression of ER stress.read more
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비만과 ER Stress
TL;DR: Endoplastic reticulum (ER) as discussed by the authors folding, lipid, sterol, and protein response (UPR) are proteins that fold, fold, and fold, respectively.
Journal ArticleDOI
A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
Madlen Stephani,Lorenzo Picchianti,Lorenzo Picchianti,Alexander Gajic,Rebecca Beveridge,Emilio Skarwan,Victor Sanchez de Medina Hernandez,Azadeh Mohseni,Marion Clavel,Yonglung Zeng,Christin Naumann,Mateusz Matuszkiewicz,Mateusz Matuszkiewicz,Eleonora Turco,Christian Loefke,Baiying Li,Gerhard Dürnberger,Gerhard Dürnberger,Michael Schutzbier,Michael Schutzbier,Hsiao Tieh Chen,Hsiao Tieh Chen,Alibek Abdrakhmanov,Adriana Savova,Khong Sam Chia,Armin Djamei,Irene Schaffner,Steffen Abel,Liwen Jiang,Karl Mechtler,Karl Mechtler,Fumiyo Ikeda,Fumiyo Ikeda,Sascha Martens,Tim Clausen,Tim Clausen,Yasin F. Dagdas +36 more
TL;DR: A cytosolic protein, C53, is identified that is specifically recruited to autophagosomes during ER-stress, in both plant and mammalian cells, and forms an ancient quality control pathway that bridges selective autophagy with ribosome-associated quality control in the ER.
Journal ArticleDOI
Protein Quality Control in Plant Organelles: Current Progress and Future Perspectives.
TL;DR: Recent progress in understanding of protein quality control in the ER, chloroplasts, and mitochondria in plants is summarized, with a focus on the common mechanisms shared in these organelles during protein homeostasis.
Journal ArticleDOI
ER-phagy responses in yeast, plants, and mammalian cells and their crosstalk with UPR and ERAD.
TL;DR: The concept of ER-phagy responses (ERPRs) was introduced in this paper. ERPRs ensure lysosomal clearance of ER portions expendable during nutrient shortage, nonfunctional, present in excess, or containing misfolded proteins.
Posted ContentDOI
A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
Madlen Stephani,Lorenzo Picchianti,Alexander Gajic,Rebecca Beveridge,Emilio Skarwan,Victor Sanchez de Medina Hernandez,Azadeh Mohseni,Marion Clavel,Yonglung Zeng,Christin Naumann,Mateusz Matuszkiewicz,Eleonora Turco,Christian Loefke,Baiying Li,Gerhard Dürnberger,Michael Schutzbier,Hsiao Tieh Chen,Alibek Abdrakhmanov,Adriana Savova,Khong Sam Chia,Armin Djamei,Irene Schaffner,Steffen Abel,Liwen Jiang,Karl Mechtler,Fumiyo Ikeda,Sascha Martens,Tim Clausen,Yasin F. Dagdas +28 more
TL;DR: A cytosolic protein, C53, is identified that is specifically recruited to autophagosomes during ER-stress, in both plant and mammalian cells, and forms an ancient quality control pathway that bridges selective autophagy with ribosome-associated quality control at the ER.
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