Journal ArticleDOI
Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray
Jia Xu,Haizhen Miao,Houfei Wu,Wensheng Huang,Rong Tang,Minyan Qiu,Jianguo Wen,Shuifang Zhu,Yao Li +8 more
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TLDR
A multiplex polymerase chain reaction (multiplex-PCR) coupled with a DNA microarray system simultaneously aiming at many targets in a consecutive reaction to detect a genetically modified organism (GMO).About:
This article is published in Biosensors and Bioelectronics.The article was published on 2006-07-15. It has received 76 citations till now. The article focuses on the topics: Detection of genetically modified organisms & Multiplex polymerase chain reaction.read more
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Testing for genetically modified organisms (GMOs): Past, present and future perspectives.
TL;DR: This paper presents an overview of GMO testing methodologies and how these have evolved and may evolve in the next decade, bearing in mind the various interests and competences of the involved stakeholders.
Journal ArticleDOI
Methods for detection of GMOs in food and feed.
Nelson Marmiroli,Elena Maestri,Mariolina Gullì,Alessio Malcevschi,Clelia Peano,Roberta Bordoni,Gianluca De Bellis +6 more
TL;DR: The GM crop regulatory framework at the international level is evaluated with reference to traceability and labelling and current analytical methods for the detection, identification, and quantification of transgenic DNA in food and feed are reviewed.
Journal ArticleDOI
New approaches in GMO detection
TL;DR: The latest progress made in GMO analysis is reviewed, taking examples from the most recently developed strategies and tools, and some of the critical aspects related to these approaches are addressed.
Journal ArticleDOI
Advances in molecular techniques for the detection and quantification of genetically modified organisms
Dimitrios S. Elenis,Despina P. Kalogianni,Kyriaki Glynou,Penelope C. Ioannou,Theodore K. Christopoulos,Theodore K. Christopoulos +5 more
TL;DR: Various types of biosensors, including surface plasmon resonance sensors, quartz crystal microbalance piezoelectric sensors, thin-film optical sensors, dry-reagent dipstick-type sensors and electrochemical sensors were introduced in GMO screening because they offer simplicity and lower cost.
Journal ArticleDOI
New trends in bioanalytical tools for the detection of genetically modified organisms: an update
TL;DR: In order to cope with the multiplicity of GMOs released onto the market, the new challenge is the development of routine detection systems for the simultaneous detection of numerous GMOs, including unknown GMOs.
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Journal ArticleDOI
Transgenic pollen harms monarch larvae
TL;DR: In a laboratory assay, it is found that larvae of the monarch butterfly, Danaus plexippus, reared on milkweed leaves dusted with pollen from Bt corn, ate less, grew more slowly and suffered higher mortality than larvae rearing on leaves dusting with untransformed corn pollen or on leaves without pollen.
Journal ArticleDOI
Detection of genetically modified organisms in foods.
TL;DR: In this article, protein- and DNA-based methods employing western blots, enzyme-linked immunosorbant assay, lateral flow strips, SouthernBlots, qualitative-, quantitative-, real-time- and limiting dilution-PCR methods, are discussed.
Journal ArticleDOI
Detection and traceability of genetically modified organisms in the food production chain
Marina Miraglia,K.G. Berdal,Carlo Brera,Philippe Corbisier,Arne Holst-Jensen,Esther J. Kok,Hans J.P. Marvin,H. Schimmel,J. Rentsch,J.P.P.F. van Rie,J. Zagon +10 more
TL;DR: Progress has been achieved in the field of sampling, detection, and traceability of genetically modified products, while some issues remain to be solved; much will depend on the threshold level for adventitious contamination set by legislation.
Journal ArticleDOI
IUPAC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder.
Markus Lipp,Brodmann P,K. Pietsch,J Pauwels,Elke Anklam,Börchers T,Braunschweiger G,U. Busch,Eklund E,Eriksen Fd,Fagan J,Fellinger A,Gaugitsch H,Hayes D,Hertel C,Hörtner H,Joudrier P,Kruse L,Meyer R,Miraglia M,Müller W,Phillipp P,B. Pöpping,Rentsch R,Wurtz A +24 more
TL;DR: Results of a collaborative trial study involving 29 laboratories in 13 countries applying a method for detecting genetically modified organisms (GMOs) in food based on using the polymerase chain reaction to determine the 35S promotor and the NOS terminator for detection of GMOs are presented.