Journal ArticleDOI
Self-reproduction of supramolecular giant vesicles combined with the amplification of encapsulated DNA.
Kensuke Kurihara,Mieko Tamura,Koh-ichiroh Shohda,Taro Toyota,Kentaro Suzuki,Tadashi Sugawara +5 more
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TLDR
The amplification of DNA is demonstrated within a self-reproducible cationic giant vesicle (host) and with the addition of a vesicular membrane precursor, this system represents a step forward in the construction of an advanced model protocell.Abstract:
The self-replication process of a giant vesicle encapsulating double-stranded DNA has been observed, which represents a supramolecular approach to the construction of a protocell. Growth and division of the vesicle occurred rapidly on addition of a membrane precursor, and amplified DNA was distributed amongst the resulting daughter giant vesicles.read more
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Journal ArticleDOI
From vesicles toward protocells and minimal cells.
TL;DR: This review focuses on both membrane-bounded (vesicular) protocells and minimal cells, and provides a membrane physics background which helps to understand how morphological transformations of vesicle systems might have happened and how vesicles reproduction might be coupled with metabolic reactions and information molecules.
Journal ArticleDOI
Biocatalytic cascades and intercommunicated biocatalytic cascades in microcapsule systems
Pu Yu Zhang,Amit Fischer,Yu Ouyang,Jianbang Wang,Yang Sung Sohn,Ola Karmi,Rachel Nechushtai,Itamar Willner +7 more
TL;DR: The guided separation and formation of dynamic supramolecular dimer microcapsular containments, and the dictated switchable operation of intercommunicated biocatalytic cascades are demonstrated.
Journal ArticleDOI
Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction
Yasuhiro Shimane,Yutetsu Kuruma +1 more
TL;DR: The quality of the giant vesicles encapsulating a cell-free protein expression system was comparable to that of the ones formed using a conventional method, in terms of the synthesis of both soluble and membrane proteins.
Journal ArticleDOI
Toward Experimental Evolution with Giant Vesicles
Hironori Sugiyama,Taro Toyota +1 more
TL;DR: This review suggests giant vesicles (GVs), which have diameters larger than 1 µm, as promising chemical cell models for studying experimental evolution, and links the concept of messy chemistry to the promising, unexplored direction of experimental evolution in GVs.
Journal ArticleDOI
A DNA Segregation Module for Synthetic Cells
TL;DR: In this article , DNA droplet segregation is obtained by cleaving the linking component between two populations of DNA Y-motifs and photolabile sites are introduced for spatio-temporally controlled DNA segregation in bulk as well as in cell-sized water-in-oil droplets and GUVs.
References
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MonographDOI
Supramolecular Chemistry: Concepts and Perspectives
TL;DR: From molecular to supramolescular chemistry: concepts and language of supramolecular chemistry, molecular recognition, information, complementarity molecular receptors - design principles and more.
Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes
TL;DR: The addition of either linear λ-phage or plasmid DNA to CLs resulted in an unexpected topological transition from liposomes to optically birefringent liquid-crystalline condensed globules, revealing a novel multilamellar structure with alternating lipid bilayer and DNA monolayers.
Journal ArticleDOI
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
TL;DR: It is shown that activated pyrimidine ribonucleotides can be formed in a short sequence that bypasses free ribose and the nucleobases, and instead proceeds through arabinose amino-oxazoline and anhydronucleoside intermediates.
Journal Article
Synthesizing life : Paths to unforeseeable science & technology
TL;DR: Advances in directed evolution and membrane biophysics make the synthesis of simple living cells, if not yet foreseeable reality, an imaginable goal.