Journal ArticleDOI
Self-reproduction of supramolecular giant vesicles combined with the amplification of encapsulated DNA.
Kensuke Kurihara,Mieko Tamura,Koh-ichiroh Shohda,Taro Toyota,Kentaro Suzuki,Tadashi Sugawara +5 more
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TLDR
The amplification of DNA is demonstrated within a self-reproducible cationic giant vesicle (host) and with the addition of a vesicular membrane precursor, this system represents a step forward in the construction of an advanced model protocell.Abstract:
The self-replication process of a giant vesicle encapsulating double-stranded DNA has been observed, which represents a supramolecular approach to the construction of a protocell. Growth and division of the vesicle occurred rapidly on addition of a membrane precursor, and amplified DNA was distributed amongst the resulting daughter giant vesicles.read more
Citations
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Journal ArticleDOI
Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies.
Yusuke Sato,Masahiro Takinoue +1 more
TL;DR: Although typical microfluidic methods are useful for the creation of artificial-cell compartments, recent methods provide further benefits, including low-cost fabrication and a reduction of the sample volume.
Journal ArticleDOI
Interface Engineering in Multiphase Systems toward Synthetic Cells and Organelles: From Soft Matter Fundamentals to Biomedical Applications.
TL;DR: Efforts to engineer liquid–liquid interfaces of multiphase systems into membrane‐bounded and membraneless compartments, which include lipid vesicles, polymer vesicle, colloidosomes, hybrids, and coacervate droplets, are summarized.
Journal ArticleDOI
Dynamic biomaterials: toward engineering autonomous feedback
TL;DR: Recent advances in nano-scale and micro-scale biomaterials, specifically artificial cells consisting of compartmentalized biochemical reactions and biologically compatible hydrogels are reviewed.
Journal ArticleDOI
Dissipative Self-Assembly of Dynamic Multicompartmentalized Microsystems with Light-Responsive Behaviors
TL;DR: This work demonstrates the autonomous generation of active polymeric systems using a strategy based on polymerization-induced self-assembly (PISA), driven by chemical fuel and exhibits a dynamic change in their multicompartmental structures when exposed to light.
Journal ArticleDOI
Streamlining genomes: toward the generation of simplified and stabilized microbial systems
TL;DR: In this article, the authors review recent progress in streamlining genomes through recombineering techniques aiming to generate insights into cellular mechanisms and responses towards the design and assembly of streamlined genome chassis together with new cellular modules in diverse biotechnological applications.
References
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MonographDOI
Supramolecular Chemistry: Concepts and Perspectives
TL;DR: From molecular to supramolescular chemistry: concepts and language of supramolecular chemistry, molecular recognition, information, complementarity molecular receptors - design principles and more.
Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes
TL;DR: The addition of either linear λ-phage or plasmid DNA to CLs resulted in an unexpected topological transition from liposomes to optically birefringent liquid-crystalline condensed globules, revealing a novel multilamellar structure with alternating lipid bilayer and DNA monolayers.
Journal ArticleDOI
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
TL;DR: It is shown that activated pyrimidine ribonucleotides can be formed in a short sequence that bypasses free ribose and the nucleobases, and instead proceeds through arabinose amino-oxazoline and anhydronucleoside intermediates.
Journal Article
Synthesizing life : Paths to unforeseeable science & technology
TL;DR: Advances in directed evolution and membrane biophysics make the synthesis of simple living cells, if not yet foreseeable reality, an imaginable goal.