Journal ArticleDOI
Self-reproduction of supramolecular giant vesicles combined with the amplification of encapsulated DNA.
Kensuke Kurihara,Mieko Tamura,Koh-ichiroh Shohda,Taro Toyota,Kentaro Suzuki,Tadashi Sugawara +5 more
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TLDR
The amplification of DNA is demonstrated within a self-reproducible cationic giant vesicle (host) and with the addition of a vesicular membrane precursor, this system represents a step forward in the construction of an advanced model protocell.Abstract:
The self-replication process of a giant vesicle encapsulating double-stranded DNA has been observed, which represents a supramolecular approach to the construction of a protocell. Growth and division of the vesicle occurred rapidly on addition of a membrane precursor, and amplified DNA was distributed amongst the resulting daughter giant vesicles.read more
Citations
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Journal ArticleDOI
Growth of Giant Peptide Vesicles Driven by Compartmentalized Transcription-Translation Activity.
TL;DR: Giant vesicles are formed using amphiphilic elastin‐like polypeptides to accommodate cell‐free gene expression reactions and a strong growth of their size is driven by changes in osmotic pressure, resulting in fusion events and uptake of membrane peptides from the environment.
Journal ArticleDOI
Do protocells preferentially retain macromolecular solutes upon division/fragmentation? A study based on the extrusion of POPC giant vesicles
TL;DR: The findings suggest that a similar mechanism operating during the critical step of vesicle growth-division could have contributed to primitive evolution.
Journal ArticleDOI
Relaxation dynamics of a compressible bilayer vesicle containing highly viscous fluid.
TL;DR: The relaxation dynamics of a compressible bilayer vesicle with an asymmetry in the viscosity of the inner and outer fluid medium is studied and the general result reduces to the previously obtained relaxation rates.
Journal ArticleDOI
Molecular simulation of protein encapsulation in vesicle formation
TL;DR: It is shown that, in this model system, the encapsulation efficiency is dominated by the interaction of the proteins with the membrane, while no significant dependence is observed on the size of the encapsulated proteins nor on the speed of the vesicle formation.
Journal ArticleDOI
Are we doing synthetic biology
Manuel Porcar,Juli Peretó +1 more
TL;DR: The state of the art on synthetic biology is reviewed and it is concluded that most research projects actually describe an extension of metabolic engineering, and the term synthetic biology should be used more sparingly.
References
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MonographDOI
Supramolecular Chemistry: Concepts and Perspectives
TL;DR: From molecular to supramolescular chemistry: concepts and language of supramolecular chemistry, molecular recognition, information, complementarity molecular receptors - design principles and more.
Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes
TL;DR: The addition of either linear λ-phage or plasmid DNA to CLs resulted in an unexpected topological transition from liposomes to optically birefringent liquid-crystalline condensed globules, revealing a novel multilamellar structure with alternating lipid bilayer and DNA monolayers.
Journal ArticleDOI
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
TL;DR: It is shown that activated pyrimidine ribonucleotides can be formed in a short sequence that bypasses free ribose and the nucleobases, and instead proceeds through arabinose amino-oxazoline and anhydronucleoside intermediates.
Journal Article
Synthesizing life : Paths to unforeseeable science & technology
TL;DR: Advances in directed evolution and membrane biophysics make the synthesis of simple living cells, if not yet foreseeable reality, an imaginable goal.