Journal ArticleDOI
Self-reproduction of supramolecular giant vesicles combined with the amplification of encapsulated DNA.
Kensuke Kurihara,Mieko Tamura,Koh-ichiroh Shohda,Taro Toyota,Kentaro Suzuki,Tadashi Sugawara +5 more
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TLDR
The amplification of DNA is demonstrated within a self-reproducible cationic giant vesicle (host) and with the addition of a vesicular membrane precursor, this system represents a step forward in the construction of an advanced model protocell.Abstract:
The self-replication process of a giant vesicle encapsulating double-stranded DNA has been observed, which represents a supramolecular approach to the construction of a protocell. Growth and division of the vesicle occurred rapidly on addition of a membrane precursor, and amplified DNA was distributed amongst the resulting daughter giant vesicles.read more
Citations
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Journal ArticleDOI
Reconstituting Natural Cell Elements in Synthetic Cells.
TL;DR: In this paper, the authors summarized recent advancements in using non-living building blocks to reconstitute some of the most crucial features of living systems in a fully engineerable chassis of a synthetic cell.
Journal ArticleDOI
Protocells programmed through artificial reaction networks.
Yifan Lyu,Yifan Lyu,Ruizi Peng,Hui Liu,Hailan Kuai,Liuting Mo,Da Han,Juan Li,Juan Li,Juan Li,Weihong Tan,Weihong Tan,Weihong Tan +12 more
TL;DR: In this review, both the milestones and recent progress of protocells programmed by artificial reaction networks are highlighted, including genetic circuits, enzyme-assisted non-genetic circuits, prebiotic mimicking reaction networks, and DNA dynamic circuits.
Journal ArticleDOI
Versatile Phospholipid Assemblies for Functional Synthetic Cells and Artificial Tissues.
TL;DR: Recent developments in the formation of versatile phospholipid assemblies are described, together with the applications of these assemblies for functional membranes, spherical and nonspherical protoorganelles, and functional synthetic cells, as well as the high‐order hierarchical structures of artificial tissues.
Journal ArticleDOI
Membrane Tension–Mediated Growth of Liposomes
TL;DR: The results show that growth of liposomes can be realized in a protein-free minimal system, which may find useful applications in achieving autonomous synthetic cells that are capable of undergoing a continuous growth-division cycle.
Journal ArticleDOI
Towards Self‐Assembled Hybrid Artificial Cells: Novel Bottom‐Up Approaches to Functional Synthetic Membranes
TL;DR: In this paper, the authors describe minimal supramolecular constructs that faithfully mimic the structure and/or function of living cells, often by controlling the assembly of highly ordered membrane architectures with defined functionality.
References
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MonographDOI
Supramolecular Chemistry: Concepts and Perspectives
TL;DR: From molecular to supramolescular chemistry: concepts and language of supramolecular chemistry, molecular recognition, information, complementarity molecular receptors - design principles and more.
Journal ArticleDOI
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,John I. Glass,Carole Lartigue,Vladimir N. Noskov,Ray-Yuan Chuang,Mikkel A. Algire,Gwynedd A. Benders,Michael G. Montague,Li Ma,Monzia Moodie,Chuck Merryman,Sanjay Vashee,Radha Krishnakumar,Nacyra Assad-Garcia,Cynthia Andrews-Pfannkoch,Evgeniya A. Denisova,Lei Young,Zhi-Qing Qi,Thomas H. Segall-Shapiro,Christopher H. Calvey,Prashanth P. Parmar,Clyde A. Hutchison,Hamilton O. Smith,J. Craig Venter +23 more
TL;DR: The design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new cells that are controlled only by the synthetic chromosome are reported.
Journal ArticleDOI
Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes
TL;DR: The addition of either linear λ-phage or plasmid DNA to CLs resulted in an unexpected topological transition from liposomes to optically birefringent liquid-crystalline condensed globules, revealing a novel multilamellar structure with alternating lipid bilayer and DNA monolayers.
Journal ArticleDOI
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions
TL;DR: It is shown that activated pyrimidine ribonucleotides can be formed in a short sequence that bypasses free ribose and the nucleobases, and instead proceeds through arabinose amino-oxazoline and anhydronucleoside intermediates.
Journal Article
Synthesizing life : Paths to unforeseeable science & technology
TL;DR: Advances in directed evolution and membrane biophysics make the synthesis of simple living cells, if not yet foreseeable reality, an imaginable goal.