Journal ArticleDOI
Silica Capsules Templated from Metal–Organic Frameworks for Enzyme Immobilization and Catalysis
TLDR
In this article, a facile method to fabricate silica (SiO2) capsules using zeolitic imidazole framework-8 nanoparticles (ZIF-8 NPs) as templates for enzyme immobilization and catalysis is reported.Abstract:
Inspired by the unique biological microenvironments of eukaryotic cells, hollow capsules are promising to immobilize enzymes due to their advantages for physical protection and improved activity of enzymes. Herein, we report a facile method to fabricate silica (SiO2) capsules using zeolitic imidazole framework-8 nanoparticles (ZIF-8 NPs) as templates for enzyme immobilization and catalysis. Enzyme-encapsulated SiO2 capsules are obtained by encapsulation of enzymes in ZIF-8 NPs and subsequent coating of silica layers, followed by the removal of templates in a mild condition (i.e., ethylenediaminetetraacetic acid (EDTA) solution). The enzyme (i.e., horseradish peroxidase, HRP) activity in SiO2 capsules is improved more than 15 times compared to that of enzyme-loaded ZIF-8 NPs. Enzymes in SiO2 capsules maintain a high relative activity after being subjected to high temperature, enzymolysis, and recycling compared to free enzymes. In addition, multienzymes (e.g., glucose oxidase and HRP) can also be coencapsulated within SiO2 capsules to show a reaction with a high cascade catalytic efficacy. This work provides a versatile strategy for enzyme immobilization and protection with potential applications in biocatalysis.read more
Citations
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Journal ArticleDOI
Multi-enzyme co-immobilized nano-assemblies: Bringing enzymes together for expanding bio-catalysis scope to meet biotechnological challenges.
Muhammad Bilal,Nazim Hussain,Juliana Heloisa Pinê Américo-Pinheiro,Yaaser Q. Almulaiky,Hafiz M.N. Iqbal +4 more
TL;DR: In this paper, the authors highlight the emergence of co-immobilization strategies by bringing multi-enzymes together with various types of nanocarriers to expand the bio-catalysis scope.
Journal ArticleDOI
Tailoring metal-organic frameworks-based nanozymes for bacterial theranostics.
TL;DR: In this paper, a review of the design and fabrication of metal-organic frameworks (MOFs) is presented, and the major obstacles and opportunities for further development in research on the interface of nanozymes and MOFs are discussed.
Journal ArticleDOI
Immobilization of Enzymes by Polymeric Materials
TL;DR: In this article, the authors reviewed three primary immobilization methods: physical adsorption, covalent binding and entrapment, with their advantages and drawbacks, and included some polymer applications and their derivatives in the immobilization of enzymes.
Journal ArticleDOI
Template-Free In Situ Encapsulation of Enzymes in Hollow Covalent Organic Framework Capsules for the Electrochemical Analysis of Biomarkers.
TL;DR: A template-free method to in situ encapsulate natural enzymes in hollow covalent organic framework (COF) capsules at room temperature, providing a new way for COFs to encapsulate enzymes and has potential applications in biocatalysis and biosensing, making artificial organelles possible.
Journal ArticleDOI
Co-delivery of enzymes and photosensitizers via metal-phenolic network capsules for enhanced photodynamic therapy
Qian Wang,Zhiliang Gao,Kaijie Zhao,Peiyu Zhang,Qi-Zhi Zhong,Qi-Zhi Zhong,Qun Yu,Shumei Zhai,Jiwei Cui +8 more
TL;DR: In this paper, a functional oxygen self-supplying and photosensitizers delivering nanosystem is fabricated via the combination of catalase (CAT), chlorin e6 (Ce6), and metalphenolic network (MPN) capsule.
References
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Industrial biocatalysis today and tomorrow
TL;DR: Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.
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TL;DR: Applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step are discussed.
Journal ArticleDOI
Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules.
Kang Liang,Raffaele Ricco,Cara M. Doherty,Mark J. Styles,Stephen Bell,Nigel Kirby,Stephen T. Mudie,David N. Haylock,Anita J. Hill,Christian J. Doonan,Paolo Falcaro +10 more
TL;DR: It is shown that proteins, enzymes and DNA rapidly induce the formation of protective metal-organic framework coatings under physiological conditions by concentrating the framework building blocks and facilitating crystallization around the biomacromolecules.
Journal ArticleDOI
One-Pot Synthesis of Protein-Embedded Metal–Organic Frameworks with Enhanced Biological Activities
TL;DR: The resulting CyT c embedded in ZIF-8 showed a 10-fold increase in peroxidase activity compared to free Cyt c in solution and thus gave convenient, fast, and highly sensitive detection of trace amounts of explosive organic peroxides in solution.
Journal ArticleDOI
Hollow‐Structured Mesoporous Materials: Chemical Synthesis, Functionalization and Applications
TL;DR: Progress in the main application aspects of HMMs, such as adsorption and storage, catalysis, and biomedicine, are discussed in detail in this article, in terms of the unique features of the combined large void space in the core and the mesoporous network in the shell.