Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: A multiplex real-time PCR assay
TLDR
A unique multiplex assay which simultaneously provides assessments of mtDNA copy number and the proportion of genomes with common large deletions by targeting two mitochondrial sites and one nuclear locus is described.Abstract:
Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: A multiplex real-time PCR assayread more
Citations
More filters
Journal ArticleDOI
HBV cccDNA: viral persistence reservoir and key obstacle for a cure of chronic hepatitis B
TL;DR: This review aims to summarise current knowledge on ccc DNA molecular biology, to highlight the experimental restrictions that have hitherto hampered faster progress and to discuss cccDNA as target for new, potentially curative therapies of chronic hepatitis B.
Journal ArticleDOI
Analysis of mtDNA/nDNA Ratio in Mice.
TL;DR: A brief and easy protocol to quantify mtDNA copy number by determining the mtDNA/nDNA ratio is described, which has been validated using a cohort of young and aged mice.
Journal ArticleDOI
Molecular characterization of breast cancer CTCs associated with brain metastasis
Debasish Boral,Monika Vishnoi,Haowen N. Liu,Wei Yin,Marc L. Sprouse,Antonio Scamardo,David S. Hong,Tuan Zea Tan,Jean P. Thiery,Jenny C. Chang,Dario Marchetti +10 more
TL;DR: It is reported that BCBM CTCs is enriched in a distinct sub-population of cells identifiable by their biomarker expression and mutational content, which may allow for early diagnosis of brain metastasis and/or help for treatment choice and its efficacy.
Journal ArticleDOI
Thinking outside the nucleus: Mitochondrial DNA copy number in health and disease
TL;DR: The existing literature which supports roles for inflammatory dynamics, immune function and alterations to cell signaling as consequences of variation in mtDNA-CN are reviewed, and it is proposed that future studies should focus on characterizing longitudinal, cell-type and cross-tissue profiles of mt DNA-CN.
Journal ArticleDOI
Human Ventricular Unloading Induces Cardiomyocyte Proliferation
Diana C. Canseco,Wataru Kimura,Sonia Garg,Shibani Mukherjee,Souparno Bhattacharya,Salim Abdisalaam,Sandeep R Das,Aroumougame Asaithamby,Pradeep P.A. Mammen,Hesham A. Sadek +9 more
TL;DR: Prolonged mechanical unloading induces adult human cardiomyocyte proliferation, possibly through prevention of mitochondria-mediated activation of DDR.
References
More filters
Journal ArticleDOI
Oxidants, antioxidants, and the degenerative diseases of aging
TL;DR: It is argued that this damage to DNA, protein, and lipid is a major contributor to aging and to degenerative diseases of aging such as cancer, cardiovascular disease, immune-system decline, brain dysfunction, and cataracts.
Journal ArticleDOI
Normal oxidative damage to mitochondrial and nuclear DNA is extensive.
TL;DR: The oxidized base, 8-hydroxydeoxyguanosine (oh8dG), 1 of approximately 20 known radiation damage products, has been assayed in the DNA of rat liver and may be responsible for the observed high mutation rate of mtDNA.
Journal ArticleDOI
Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA damage in human cells following oxidative stress
TL;DR: Results indicate that mitochondrial DNA is a critical cellular target for ROS, and persistent mitochondrial DNA damage may serve as a useful biomarker for ROS-associated diseases.
Journal ArticleDOI
The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences.
TL;DR: The Sequence Manipulation Suite is a collection of freely available JavaScript applications for molecular biologists that consists of over 30 utilities for analyzing and manipulating sequence data, including the following.
Journal ArticleDOI
Mitochondrial abnormalities in Alzheimer's disease.
Keisuke Hirai,Gjumrakch Aliev,Akihiko Nunomura,Akihiko Nunomura,Hisashi Fujioka,Robert L. Russell,Craig S. Atwood,Anne B. Johnson,Yvonne Kress,Harry V. Vinters,Massimo Tabaton,Shun Shimohama,Adam D. Cash,Sandra L. Siedlak,Peggy L.R. Harris,Paul K. Jones,Robert B. Petersen,George Perry,Mark A. Smith +18 more
TL;DR: Morphometric analysis showed that mitochondria are significantly reduced in Alzheimer's disease, and the relationship shown here between the site and extent of mitochondrial abnormalities and oxidative damage suggests an intimate and early association between these features in dementia.
Related Papers (5)
Number matters: control of mammalian mitochondrial DNA copy number
Is mitochondrial DNA content a potential biomarker of mitochondrial dysfunction
Afshan N. Malik,Anna Czajka +1 more