Journal ArticleDOI
Studying the Response of Aortic Endothelial Cells under Pulsatile Flow Using a Compact Microfluidic System.
Mokhaled Mohammed,Peter Thurgood,Christopher Gilliam,Ngan Nguyen,Elena Pirogova,Karlheinz Peter,Khashayar Khoshmanesh,Sara Baratchi +7 more
TLDR
A piezoelectric pumping system for studying the mechanobiology of human aortic endothelial cells (HAECs) under pulsatile flow in microfluidic structures and the effect of atheroprone and atheroprotective pulsatile shear stress on endothelial cytoskeleton remodeling, distribution of β-catenin as well as nuclear shape and size is investigated.Abstract:
We describe a piezoelectric pumping system for studying the mechanobiology of human aortic endothelial cells (HAECs) under pulsatile flow in microfluidic structures. The system takes advantage of commercially available components, including pumps, flow sensors, and microfluidic channels, which can be easily integrated, programmed, and operated by cellular biologists. Proof-of-concept experiments were performed to elucidate the complex mechanotransduction processes of endothelial cells to pulsatile flow. In particular, we investigated the effect of atheroprone and atheroprotective pulsatile shear stress on endothelial cytoskeleton remodeling and distribution of β-catenin, as well as nuclear shape and size. The system is simple to operate, relatively inexpensive, portable, and controllable, providing opportunities for studying the mechanobiology of endothelial cells using microfluidic technologies.read more
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Wearable sensors: At the frontier of personalised health monitoring, smart prosthetics and assistive technologies
TL;DR: Wearable sensors have evolved from body-worn fitness tracking devices to multifunctional, highly integrated, compact, and versatile sensors which can be mounted onto the desired locations of our clothes or body to continuously monitor our body signals, and better interact and communicate with our surrounding environment or equipment as discussed by the authors.
Journal ArticleDOI
Transcatheter Aortic Valve Implantation Represents an Anti-Inflammatory Therapy Via Reduction of Shear Stress-Induced, Piezo-1-Mediated Monocyte Activation.
Sara Baratchi,Sara Baratchi,Maria T.K. Zaldivia,Maria Wallert,Julia Loseff-Silver,Sefaa Al-aryahi,Jalal Zamani,Peter Thurgood,Agus Salim,Agus Salim,Nay M. Htun,Nay M. Htun,Dion Stub,Dion Stub,Dion Stub,Parisa Vahidi,Stephen J. Duffy,Antony Walton,Thanh H Nguyen,Anthony Jaworowski,Khashayar Khoshmanesh,Karlheinz Peter,Karlheinz Peter,Karlheinz Peter +23 more
TL;DR: It is demonstrated that high shear stress, as present in patients with aortic valve stenosis, activates multiple monocyte functions, and Piezo-1 is identified as the mainly responsible mechanoreceptor, representing a potentially druggable target.
Journal ArticleDOI
Microfluidic Skin-on-a-Chip Models: Toward Biomimetic Artificial Skin.
TL;DR: The present Review collates the progress of SoC platforms with a focus on their fabrication and the incorporation of mechanical cues, sensors, and blood vessels.
Journal ArticleDOI
Open-source, 3D-printed Peristaltic Pumps for Small Volume Point-of-Care Liquid Handling
Michael R. Behrens,Haley C. Fuller,Emily R. Swist,Jingwen Wu,Md. Mydul Islam,Zhicheng Long,Warren C. Ruder,Robert L. Steward +7 more
TL;DR: This work presents a low-cost, open-source peristaltic pump constructed with a combination of three dimensional (3D)-printed parts and common hardware, which is amenable to deployment with microfluidic devices for point-of-care diagnostics.
Journal ArticleDOI
Reconfigurable, Self-Sufficient Convective Heat Exchanger for Temperature Control of Microfluidic Systems.
Jiu Yang Zhu,Sergio Aguilera Suarez,Peter Thurgood,Ngan Nguyen,Mokhaled Mohammed,Haneen Abdelwahab,Scott Needham,Elena Pirogova,Kamran Ghorbani,Sara Baratchi,Khashayar Khoshmanesh +10 more
TL;DR: The versatility, simplicity and self-sufficiency of the heat exchanger makes it suitable for various microfluidic based cellular assays.
References
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Journal ArticleDOI
Monolithic microfabricated valves and pumps by multilayer soft lithography
TL;DR: An extension to the soft lithography paradigm, multilayersoft lithography, with which devices consisting of multiple layers may be fabricated from soft materials is described, to build active microfluidic systems containing on-off valves, switching valves, and pumps entirely out of elastomer.
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CircStat: A Matlab Toolbox for Circular Statistics
TL;DR: The CircStat toolbox for MATLAB is implemented which provides methods for the descriptive and inferential statistical analysis of directional data and analyzes a dataset from neurophysiology to demonstrate the capabilities of the Circstat toolbox.
Journal ArticleDOI
The many faces and functions of β‐catenin
TL;DR: One focus will be the interaction of β‐catenin with different transcription factors and the potential implications of these interactions for direct cross‐talk between β‐ catenin and non‐Wnt signalling pathways.
Journal ArticleDOI
Laminar Shear Stress Mechanisms by Which Endothelial Cells Transduce an Atheroprotective Force
Oren Traub,Bradford C. Berk +1 more
TL;DR: The ability of shear stress to modulate atherogenesis via its effects on endothelial-mediated alterations in coagulation, leukocyte and monocyte migration, smooth muscle growth, lipoprotein uptake and metabolism, and endothelial cell survival is explored.
Journal ArticleDOI
Mechanotransduction in vascular physiology and atherogenesis
Cornelia Hahn,Martin A. Schwartz +1 more
TL;DR: This work has highlighted the potential endothelial mechanotransducers that might mediate responses to blood flow, the effects of atheroprotective rather than atherogenic flow,The mechanisms that contribute to the progression of the disease and how systemic factors interact with flow patterns to cause atherosclerosis.