Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
TLDR
A high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores that can, in principle, reach molecular-scale resolution is developed.Abstract:
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.read more
Citations
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Journal ArticleDOI
Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes†
Mike Heilemann,Sebastian van de Linde,Mark Schüttpelz,Robert Kasper,Britta Seefeldt,Anindita Mukherjee,Philip Tinnefeld,Markus Sauer +7 more
Journal ArticleDOI
Spatially resolved, highly multiplexed RNA profiling in single cells
Kok Hao Chen,Alistair N. Boettiger,Jeffrey R. Moffitt,Siyuan Wang,Xiaowei Zhuang,Xiaowei Zhuang +5 more
TL;DR: This report reports multiplexed error-robust FISH (MERFISH), a single-molecule imaging method that allows thousands of RNA species to be imaged in single cells by using combinatorial FISH labeling with encoding schemes capable of detecting and/or correcting errors.
Journal ArticleDOI
Super-Resolution Fluorescence Microscopy
TL;DR: It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
Journal ArticleDOI
Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
TL;DR: A family of photo-switchable fluorescent probes is introduced and multicolor stochastic optical reconstruction microscopy (STORM) is demonstrated, to facilitate direct visualization of molecular interactions at the nanometer scale.
Journal ArticleDOI
Spiropyran-based dynamic materials
TL;DR: This review discusses the synthesis, switching conditions, and use of dynamic materials in which spiropyran has been attached to the surfaces of polymers, biomacromolecules, inorganic nanoparticles, as well as solid surfaces.
References
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