Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
TLDR
A high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores that can, in principle, reach molecular-scale resolution is developed.Abstract:
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.read more
Citations
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ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging
TL;DR: ThunderSTORM is an open-source, interactive and modular plug-in for ImageJ designed for automated processing, analysis and visualization of data acquired by single-molecule localization microscope methods such as photo-activated localization microscopy and stochastic optical reconstruction microscopy.
Journal ArticleDOI
Revitalizing membrane rafts: new tools and insights
Kai Simons,Mathias J. Gerl +1 more
TL;DR: How the field has matured and an evolving model in which membranes are occupied by fluctuating nanoscale assemblies of sphingolipids, cholesterol and proteins that can be stabilized into platforms that are important in signalling, viral infection and membrane trafficking are presented.
Journal ArticleDOI
Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Lothar Schermelleh,Peter M. Carlton,Sebastian Haase,Lin Shao,Lukman Winoto,Peter Kner,Brian Burke,M. Cristina Cardoso,David A. Agard,Mats G. L. Gustafsson,Heinrich Leonhardt,John W. Sedat +11 more
TL;DR: Three-dimensional structured illumination microscopy (3D-SIM) opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
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Surface-Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges
TL;DR: An outlook of the key challenges in bioanalytical SERS, including reproducibility, sensitivity, and spatial and time resolution is given.
References
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TL;DR: A family of concepts has emerged that overcomes the diffraction barrier altogether and, relying on saturated optical transitions, these concepts are limited only by the attainable saturation level.
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