Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs
Adrian E. Platts,David J. Dix,Héctor E. Chemes,Kary E. Thompson,Robert Goodrich,John C Rockett,Vanesa Y. Rawe,Silvina Quintana,Michael P. Diamond,Lillian F. Strader,Stephen A. Krawetz +10 more
TLDR
A cross-platform microarray strategy was used to assess the profile of human spermatozoal transcripts from fertile males who had fathered at least one child compared to teratozoospermic individuals and revealed the transcriptional perturbation common to the affected individuals.Abstract:
We are coming to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suite of structures, proteins and RNAs. Although the role of some of the structures and proteins as requisite elements for early human development has been established, the function of the sperm-delivered RNAs remains a point for discussion. The presence of RNAs in transcriptionally quiescent spermatozoa can only be derived from transcription that precedes late spermiogenesis. A cross-platform microarray strategy was used to assess the profile of human spermatozoal transcripts from fertile males who had fathered at least one child compared to teratozoospermic individuals. Unsupervised clustering of the data followed by pathway and ontological analysis revealed the transcriptional perturbation common to the affected individuals. Transcripts encoding components of various cellular remodeling pathways, such as the ubiquitin-proteosome pathway, were severely disrupted. The origin of the perturbation could be traced as far back as the pachytene stage of spermatogenesis. It is anticipated that this diagnostic strategy will prove valuable for understanding male factor infertility.read more
Citations
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The biology of infertility: research advances and clinical challenges
Martin M. Matzuk,Dolores J. Lamb +1 more
TL;DR: The past six years have witnessed a virtual explosion in the identification of gene mutations or polymorphisms that cause or are linked to human infertility, but translation of these findings to the clinic remains slow, however, as do new methods to diagnose and treat infertile couples.
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The genetic causes of male factor infertility: A review
TL;DR: Analysis of the genetic factors that impact male factor infertility will provide valuable insights into the creation of targeted treatments for patients and the determination of the causes of idiopathic infertility.
Journal ArticleDOI
Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel
TL;DR: By successfully patch clamping human spermatozoa, it is shown that proton channel Hv1 is their dominant proton conductance, which makes it an attractive target for controlling male fertility.
Journal ArticleDOI
The presence, role and clinical use of spermatozoal RNAs
TL;DR: Dad delivers a complex population of RNAs to the oocyte at fertilization that likely influences fertilization, embryo development, the phenotype of the offspring and possibly future generations.
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