Journal ArticleDOI
Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat
TLDR
It is concluded that chlorpyrifos induces severe testicular damage and results in reduction in sperm count and thus affect fertility, and application of such insecticide should be limited to a designed programme.Abstract:
Organophosphates are among the most widely used synthetic insect pesticides. The widespread use of organophosphates has stimulated research into the possible existence of effects related with their reproductive toxic activity. Present study was therefore, undertaken to assess the effects of chlorpyrifos on testes, the main organ of male reproduction. Chlorpyrifos at the dose levels of 7.5, 12.5 and 17.5 mg/kg b. wt./day was administered orally to male rats of Wistar strain for 30 days to evaluate the toxic alterations in testicular histology, biochemistry, sperm dynamics and testosterone levels. The body weight of animals did not show any significant change, however, a significant reduction was observed in testes. Chlorpyrifos also brought about marked reduction in epididymal and testicular sperm counts in exposed males and a decrease in serum testosterone concentration. Histopathological examination of testes showed mild to severe degenerative changes in seminiferous tubules at various dose levels. Fertility test showed 85% negative results. A significant reduction in the sialic acid content of testes and testicular glycogen was noticed, whereas the protein and cholesterol content was raised at significant levels. All these toxic effects are moderate at low doses and become severe at higher dose levels. From the results of the present study it is concluded that chlorpyrifos induces severe testicular damage and results in reduction in sperm count and thus affect fertility. Small changes in sperm counts are known to have adverse affects on human fertility. Therefore, application of such insecticide should be limited to a designed programme.read more
Citations
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Journal ArticleDOI
Occupational exposure to pesticides and consequences on male semen and fertility: A review
TL;DR: Effect of pesticides on sperm quality is undeniable, well-designed long-term studies are needed to elucidate all the possible affecting variables such as socioeconomic, cultural, nutritional, occupational, physical, and clinical characteristics alongside pesticides.
Journal ArticleDOI
Chlorpyrifos induced hepatotoxic and hematologic changes in rats: the role of quercetin and catechin.
Fatma Gokce Uzun,Yusuf Kalender +1 more
TL;DR: Catechin and quercetin significantly reduce chlorpyrifos induced hepatotoxicity in rats as a result of protective effects on examining parameters but not completely.
Journal ArticleDOI
Pesticide chlorpyrifos acts as an endocrine disruptor in adult rats causing changes in mammary gland and hormonal balance
Clara Ventura,María Rosa Ramos Nieto,Nadia Bourguignon,Victoria Lux-Lantos,Horacio A. Rodríguez,Gabriel Cao,Andrea Randi,Claudia Cocca,Mariel Núñez +8 more
TL;DR: These findings warn about the harmful effects that CPF exerts on mammary gland, suggesting that this compound may act as a risk factor for breast cancer.
Journal ArticleDOI
Protective properties of 6-gingerol-rich fraction from Zingiber officinale (Ginger) on chlorpyrifos-induced oxidative damage and inflammation in the brain, ovary and uterus of rats.
Amos O. Abolaji,Mercy Ojo,Tosin T. Afolabi,Mary D. Arowoogun,Darlinton Nwawolor,Ebenezer O. Farombi +5 more
TL;DR: Overall, the protective effects of 6-GRF on CPF-induced toxicity in the brain and reproductive organs of rats may be due to its potent antioxidative, anti-inflammatory and antiapoptotic properties.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
A new method for the direct determination of serum cholesterol.
TL;DR: Precision and reproducibility are demonstrated as well as the absorption characteristics of the purple color produced through the spectral range of 400 to 700 mμ.
Book
Statistics for toxicologists
TL;DR: In this article, the authors explain how the computer programs work and why and when they can be applied to problems in toxicology, and discuss the statistical models used and their applications in a general fashion.