Journal ArticleDOI
The emergence of optical elastography in biomedicine
TLDR
Optical elastography, the use of optics to characterize and map the mechanical properties of biological tissue, involves measuring the deformation of tissue in response to a load.Abstract:
Optical elastography, the use of optics to characterize and map the mechanical properties of biological tissue, involves measuring the deformation of tissue in response to a load. Such measurements may be used to form an image of a mechanical property, often elastic modulus, with the resulting mechanical contrast complementary to the more familiar optical contrast. Optical elastography is experiencing new impetus in response to developments in the closely related fields of cell mechanics and medical imaging, aided by advances in photonics technology, and through probing the microscale between that of cells and whole tissues. Two techniques — optical coherence elastography and Brillouin microscopy — have recently shown particular promise for medical applications, such as in ophthalmology and oncology, and as new techniques in cell mechanics.read more
Citations
More filters
Journal ArticleDOI
Evaluating biomechanical properties of murine embryos using Brillouin microscopy and optical coherence tomography.
Raksha Raghunathan,Jitao Zhang,Chen Wu,Justin Rippy,Manmohan Singh,Kirill V. Larin,Kirill V. Larin,Giuliano Scarcelli +7 more
TL;DR: This work proposes the use of Brillouin microscopy in combination with optical coherence tomography (OCT) to measure stiffness as well as structural changes in a developing embryo.
Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy
Giuliano Scarcelli,Kripa Patel,William J. Polacheck,Hadi Tavakoli Nia,Alan J. Grodzinsky,Roger D. Kamm,Seok Hyun Yun +6 more
TL;DR: In this article, the authors developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution.
Journal ArticleDOI
Background-deflection Brillouin microscopy reveals altered biomechanics of intracellular stress granules by ALS protein FUS.
Giuseppe Antonacci,Valeria de Turris,Alessandro Rosa,Alessandro Rosa,Giancarlo Ruocco,Giancarlo Ruocco +5 more
TL;DR: A non-contact and label-free imaging method, named background-deflection Brillouin microscopy, is presented, enabling the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells at a sub-micron resolution.
Journal ArticleDOI
Brillouin microscopy: assessing ocular tissue biomechanics.
Seok Hyun Yun,Dimitri Chernyak +1 more
TL;DR: Brillouin microscopy is a promising technology under commercial development at present that enables physicians to characterize the biomechanical properties of ocular tissues.
Journal ArticleDOI
Parametric imaging of attenuation by optical coherence tomography: review of models, methods, and clinical translation.
Peijun Gong,Mitra Almasian,Gijs van Soest,Daniel M. de Bruin,Ton G. van Leeuwen,David D. Sampson,David D. Sampson,Dirk J. Faber +7 more
TL;DR: A review of the main models and methods used for the determination of the OCT attenuation coefficient, together with a survey of preclinical and clinical demonstrations and their translation potential, hopes to provide a basis for discussion toward establishing a consensus.
References
More filters
Journal ArticleDOI
Optical coherence tomography
David Huang,Eric A. Swanson,Charles P. Lin,Joel S. Schuman,William G. Stinson,Warren Chang,Michael R. Hee,Thomas J. Flotte,Kenton W. Gregory,Carmen A. Puliafito,James G. Fujimoto +10 more
TL;DR: OCT as discussed by the authors uses low-coherence interferometry to produce a two-dimensional image of optical scattering from internal tissue microstructures in a way analogous to ultrasonic pulse-echo imaging.
Book ChapterDOI
Optical Coherence Tomography
Eric A. Swanson,J. Izatt,M. Bee,David Huang,Charles P. Lin,Carmen A. Puliafito,Carmen A. Puliafito,James G. Fujimoto +7 more
TL;DR: Optical coherence tomography (OCT) has developed rapidly since its first realisation in medicine and is currently an emerging technology in the diagnosis of skin disease as mentioned in this paper, where OCT is an interferometric technique that detects reflected and backscattered light from tissue.
Journal ArticleDOI
Performance of fourier domain vs. time domain optical coherence tomography.
TL;DR: It is shown that FDOCT systems have a large sensitivity advantage and allow for sensitivities well above 80dB, even in situations with low light levels and high speed detection.
Journal ArticleDOI
Shear wave elasticity imaging: a new ultrasonic technology of medical diagnostics
TL;DR: A physical and mathematical basis of SWEI is presented and some experimental results of pilot studies proving feasibility of this new ultrasonic technology are presented, including a theoretical model of shear oscillations in soft biological tissue remotely induced by the radiation force of focused ultrasound.
Journal ArticleDOI
Quantitative assessment on the cloning efficiencies of lentiviral transfer vectors with a unique clone site.
Gang Zhang,Anurag Tandon +1 more
TL;DR: A combinatorial strategy to efficiently construct LVs using EGFP, hPlk2 wild type (WT) and mutant genes as inserts using BamH I site for the inserts and the amounts and ratios of the insert and vector DNA were optimized to increase monomeric ligation.
Related Papers (5)
Optical coherence elastography – OCT at work in tissue biomechanics [Invited]
Kirill V. Larin,David D. Sampson +1 more
Confocal Brillouin microscopy for three-dimensional mechanical imaging
Giuliano Scarcelli,Seok Hyun Yun +1 more