Journal ArticleDOI
The functional roles of mammalian DNA polymerase.
TLDR
A full understanding of the function of the eucaryotic DNA polymerases now depends on the clarification of the intimate mechanisms of DNA synthesis in various modes as discussed by the authors, and identification of the accessory proteins, cofactors, and replicative DNA structures within a given replication complex, as has been accomplished in certain procaryotes (53), may help in this task.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1979-12-01. It has received 98 citations till now. The article focuses on the topics: DNA polymerase II & DNA clamp.read more
Citations
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Journal ArticleDOI
Use of a low-speed, iso-density percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations.
B. L. Kreamer,Jeffrey L. Staecker,Norimasa Sawada,Gerald L. Sattler,M. T. S. Hsia,Henry C. Pitot +5 more
TL;DR: The enriched parenchymal cells have been shown to be superior to untreated cells by a number of criteria including: preparation homogeneity, cell morphology, maintenance of cytochrome P-450, hormonal responsiveness, and the DNA repair capability after treatment with benzo[a]pyrene or 2-acetylaminofluorene.
Journal ArticleDOI
DNA polymerase γ, the mitochondrial replicase
TL;DR: The two-subunit holoenzyme is an efficient and processive polymerase, which exhibits high fidelity in nucleotide selection and incorporation while proofreading errors with its intrinsic 3′ → 5′ exonuclease.
Journal ArticleDOI
The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations.
TL;DR: The frequency and specificity of mutations produced in vitro by eucaryotic DNA polymerase-beta have been determined in a forward mutation assay using a 250-base target sequence in M13mp2 DNA, indicating that purified DNA polymerases alone are capable of producing deletions.
Journal ArticleDOI
The Translesion DNA Polymerase ζ Plays a Major Role in Ig and bcl-6 Somatic Hypermutation
Hong Zan,Atsumasa Komori,Zongdong Li,Andrea Cerutti,András Schaffer,Martin F. Flajnik,Marilyn Diaz,Paolo Casali +7 more
TL;DR: Inhibition of the pol zeta REV3 catalytic subunit by specific phosphorothioate-modified oligonucleotides impaired Ig and bcl-6 hypermutation and UV damage-induced DNA mutagenesis, without affecting cell cycle or viability.
Journal ArticleDOI
The mutational specificity of DNA polymerases-alpha and -gamma during in vitro DNA synthesis.
TL;DR: A correlation between the accuracy and processivity of DNA polymerization and the most accurate DNA polymerase also incorporates the most nucleotides per association with the primer-template, while the least accurate enzyme (pol-beta) is the least processive is demonstrated.
References
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Journal ArticleDOI
On the mechanism of DNA replication in mammalian chromosomes
Joel A. Huberman,Arthur D. Riggs +1 more
TL;DR: It is found that the long fibers of which chromosomal DNA is composed are made up of many tandemly joined sections in each of which DNA is replicated at a fork-like growing point.
Journal ArticleDOI
Specific Gene Amplification in Oocytes Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA
Donald D. Brown,Igor B. Dawid +1 more
Journal ArticleDOI
DNA polymerase associated with human hepatitis B antigen.
P M Kaplan,P M Kaplan,P M Kaplan,Richard L. Greenman,Richard L. Greenman,Richard L. Greenman,John L. Gerin,John L. Gerin,John L. Gerin,Robert H. Purcell,Robert H. Purcell,Robert H. Purcell,William S. Robinson,William S. Robinson,William S. Robinson +14 more
TL;DR: DNA polymerase activity was detected in each of eight preparations of concentrated human hepatitis B antigen (HBAg) rich in Dane particles prepared by high-speed centrifugation of antigen-positive human plasma and in none of seven control preparations prepared in the same way from HBAg-negative plasma.
Journal ArticleDOI
Evidence for the binding of polynuclear aromatic hydrocarbons to the nucleic acids of mouse skin: relation between carcinogenic power of hydrocarbons and their binding to deoxyribonucleic acid.
Peter Brookes,P. D. Lawley +1 more
TL;DR: Evidence for the Binding of Polynuclear Aromatic Hydrocarbons to the Nucleic Acids of Mouse Skin : Relation between Carcinogenic Power of hydrocarbons and their Binding to Deoxyribonucleic Acid is presented.
Journal ArticleDOI
Benzo(a)pyrene diol epoxides as intermediates in nucleic acid binding in vitro and in vivo.
I. B. Weinstein,Alan M. Jeffrey,K. W. Jennette,S. H. Blobstein,Ronald G. Harvey,Chris Harris,Herman Autrup,Hiroshi Kasai,Koji Nakanishi +8 more
TL;DR: Evidence has been obtained that a specific isomer of a diol epoxide derivative of benzo(a)pyrene, (+/-)-7 beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo( a)pyene, is an intermediate in the binding of benzos(a).pyrene to RNA in cultured bovine bronchial mucosa.