The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation.
Nevan J. Krogan,Jim Dover,Adam Wood,Jessica Schneider,Jonathan Heidt,Marry Ann Boateng,Kimberly Dean,Owen Ryan,Ashkan Golshani,Mark Johnston,Jack Greenblatt,Ali Shilatifard +11 more
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TLDR
It is reported that the Paf1 protein complex, which is associated with the elongating RNA polymerase II, is required for methylation of lysines 4 and 79 of histone H3 and for silencing of expression of a telomere-associated gene.About:
This article is published in Molecular Cell.The article was published on 2003-03-01 and is currently open access. It has received 751 citations till now. The article focuses on the topics: Histone methyltransferase & Histone methylation.read more
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Journal ArticleDOI
The Role of Chromatin during Transcription
TL;DR: This Review highlights advances in the understanding of chromatin regulation and discusses how such regulation affects the binding of transcription factors as well as the initiation and elongation steps of transcription.
PatentDOI
Histone demethylation mediated by the nuclear amine oxidase homolog lsd1
Yang Shi,Yujiang Shi +1 more
TL;DR: In this paper, the authors identify a histone demethylase conserved from S. pombe to human and reveal dynamic regulation of histone methylation by both histonemethylases and demethylases.
Journal ArticleDOI
A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells
TL;DR: The results of a genome-wide analysis of human cells suggest that most protein-coding genes, including most genes thought to be transcriptionally inactive, experience transcription initiation, and that transcription initiation at most genes is a general phenomenon in human cells.
Journal ArticleDOI
Histone methylation: a dynamic mark in health, disease and inheritance
Eric L. Greer,Yang Shi +1 more
TL;DR: This work provides a broad overview of how histone methylation is regulated and leads to biological outcomes and suggests its links to disease and ageing and possibly to transmission of traits across generations are illustrated.
Journal ArticleDOI
Genomic Maps and Comparative Analysis of Histone Modifications in Human and Mouse
Bradley E. Bernstein,Bradley E. Bernstein,Bradley E. Bernstein,Michael Kamal,Kerstin Lindblad-Toh,Stefan Bekiranov,Dione K. Bailey,Dana J. Huebert,Dana J. Huebert,Scott McMahon,Scott McMahon,Elinor K. Karlsson,Edward J. Kulbokas,Thomas R. Gingeras,Stuart L. Schreiber,Stuart L. Schreiber,Eric S. Lander,Eric S. Lander +17 more
TL;DR: Methylation patterns at orthologous loci are strongly conserved between human and mouse even though many methylated sites do not show sequence conservation notably higher than background, which suggests that the DNA elements that direct the methylation represent only a small fraction of the region or lie at some distance from the site.
References
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Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
Amy Hin Yan Tong,Marie Evangelista,Ainslie B. Parsons,Hong Xu,Gary D. Bader,Gary D. Bader,Nicholas Pagé,Mark D. Robinson,Sasan Raghibizadeh,Christopher W. V. Hogue,Christopher W. V. Hogue,Howard Bussey,Brenda J. Andrews,Mike Tyers,Mike Tyers,Charles Boone,Charles Boone +16 more
TL;DR: A method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants is developed, which should produce a global map of gene function.
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Active genes are tri-methylated at K4 of histone H3
Helena Santos-Rosa,Robert Schneider,Andrew J. Bannister,Julia A. Sherriff,Bradley E. Bernstein,N. C. Tolga Emre,Stuart L. Schreiber,Jane Mellor,Tony Kouzarides +8 more
TL;DR: It is shown that the Saccharomyces cerevisiae Set1 protein can catalyse di- and tri-methylation of K4 and stimulate the activity of many genes, establishing the concept of methyl status as a determinant for gene activity and extending considerably the complexity of histone modifications.
Journal ArticleDOI
Alteration of Nucleosome Structure as a Mechanism of Transcriptional Regulation
TL;DR: Recently, proteins that were initially identified as necessary for transcriptional regulation have been shown to alter nucleosomal structure and are likely to play a central role in appropriate regulation of eukaryotic genes.
Journal ArticleDOI
Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast
Zu-Wen Sun,C. David Allis +1 more
TL;DR: It is shown that the ubiquitin-conjugating enzyme Rad6 (Ubc2) mediates methylation of histone H3 at lysine 4 (Lys 4) through ubiquitination of H2B at Lys 123 in yeast (Saccharomyces cerevisiae) to reveal a pathway leading to gene regulation through concerted histone modifications on distinct histone tails.
Journal ArticleDOI
Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription
TL;DR: A dynamic association of mRNA processing factors with differently modified forms of the polymerase throughout the transcription cycle is suggested.
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