The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
More filters
Journal ArticleDOI
Purification, crystallization and preliminary X-ray crystallographic analysis of PBP4 from Listeria monocytogenes.
TL;DR: In this study, a class A PBP4 from Listeria monocytogenes was overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method, resulting in moenomycin-derived antibiotics possessing broad-spectrum activity.
Dissertation
Muropeptide recycling in Bacillus subtilis : beta-N-acetylglucosaminidase NagZ operates by a unique Asp-His catalytic dyad mechanism
TL;DR: The muropeptide catabolic pathway presented here is the first evidence forcell wall recycling in a Gram-positive organism that proceeds distinct from the cell wall recycling pathway of E. coli and other Gram-negative bacteria.
Dissertation
Secondary Metabolites from Fungi: Natural Product Diversity, Structure Elucidation and Bioactivity
TL;DR: This dissertation describes secondary metabolites isolated from different fungi, including Trichoderma harzianum, Aspergillus terreus, Penicillium sp.
Mechanisms and Dynamics of Mecillinam Resistance in Escherichia coli
TL;DR: The introduction of antibiotics in healthcare is one of the most important medical achievements with regard to reducing human morbidity and mortality, however, bacterial pathogens have acquired antimalarial properties.
Penicillin Binding Proteins: An Insight Into Novel Antibacterial Drug Target
Pallavi Sahare,Archana Moon +1 more
TL;DR: Detailed insight is focused on on PBP classification and mechanism, thus opening avenues for an effective and novel antibacterial drug target research and therapy.
References
More filters
Journal ArticleDOI
Peptidoglycan types of bacterial cell walls and their taxonomic implications.
Book
Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.