The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
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The Role of Elyc Protein in Protection Against Oxidative Stress in E. Coli Cells
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Dissertation
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Posted ContentDOI
A cytoskeletal network maintains filament shape in the multicellular cyanobacterium Anabaena sp. PCC 7120
Benjamin L. Springstein,Dennis J. Nürnberg,Ann-Katrin Kieninger,Christian Woehle,Julia Weissenbach,Marius L. Theune,Andreas O. Helbig,Andreas Tholey,Iris Maldener,Tal Dagan,Karina Stucken +10 more
TL;DR: This work proposes that this cytoskeletal network is essential for the manifestation of the linear filament phenotype in Anabaena and indicates that the four CCRPs form a proteinaceous network that stabilizes the AnabaENA multicellular filament.
Journal ArticleDOI
Molecular modeling of ceftriaxone activation in the active sites of penicillin-binding proteins 2
TL;DR: The 2D maps of the Laplacian of electron density show a more efficient activation of the substrate by the wild type enzyme rather than mutated species, consistent with the geometry features: distributions of the lengths of hydrogen bonds forming oxyanion hole and nucleophilic attack distance that are shifted toward lower values.
References
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Journal ArticleDOI
Peptidoglycan types of bacterial cell walls and their taxonomic implications.
Book
Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.