The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
Reads0
Chats0
TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
More filters
Journal ArticleDOI
Host-Polarized Cell Growth in Animal Symbionts
Nika Pende,Jinglan Wang,Philipp M. Weber,Jolanda Verheul,Erkin Kuru,Simon K.-M. R. Rittmann,Nikolaus Leisch,Michael S. VanNieuwenhze,Yves V. Brun,Tanneke den Blaauwen,Silvia Bulgheresi +10 more
TL;DR: It is concluded that MreB function is required for symbiont widening and division, and this data invoke a reassessment of the localization and function of the bacterial actin homolog.
Journal ArticleDOI
The Role of Treponema denticola Motility in Synergistic Biofilm Formation With Porphyromonas gingivalis
Hong Min Ng,Nada Slakeski,Catherine A. Butler,Paul D. Veith,Yu-Yen Chen,Sze Wei Liu,Brigitte Hoffmann,Stuart G. Dashper,Eric C. Reynolds +8 more
TL;DR: A 5-fold reduction in dual-species biofilm biomass was found with the motility-specific ΔflgE mutant suggesting that T. denticola periplasmic flagella are essential in synergistic biofilm formation with P. gingivalis.
Journal ArticleDOI
β-Lactam antibiotic targets and resistance mechanisms: from covalent inhibitors to substrates.
TL;DR: This tutorial-style review of the β-lactam antibiotics provides an overview of their covalent interactions with their target proteins and resistance mechanisms, and introduces the l,d-transpeptidases, a group of bacterial enzymes involved in peptidoglycan synthesis which are also targeted by β- lactams.
Journal ArticleDOI
Common Cell Shape Evolution of Two Nasopharyngeal Pathogens
Frédéric J. Veyrier,Nicolas Biais,Pablo Morales,Nouria Belkacem,Cyril Guilhen,Sylvia L. Ranjeva,Odile Sismeiro,Gérard Pehau-Arnaudet,Eduardo P. C. Rocha,Catherine Werts,Muhamed-Kheir Taha,Ivo G. Boneca +11 more
TL;DR: A strong selection of genetic events during the colonization of the nasopharynx may have been forced by the requirement of evolving permissive interaction with the immune system, the need to reduce the cellular surface exposed to immune attacks without reducing the intracellular storage capacity, or the necessity to better compete for adhesion to target cells.
Journal ArticleDOI
Penicillin-Binding Protein Imaging Probes
Ozden Kocaoglu,Erin E. Carlson +1 more
TL;DR: A general protocol to label and detect subsets of active PBPs in live, Gram‐positive bacteria using fluorescent β‐lactams and fluorophore‐conjugated analogs is described.
References
More filters
Journal ArticleDOI
Peptidoglycan types of bacterial cell walls and their taxonomic implications.
Book
Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.