The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
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TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
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Determinants of Bacterial Morphology: From Fundamentals to Possibilities for Antimicrobial Targeting.
TL;DR: The molecular determinants underlying morphology are reviewed, the evidence linking bacterial morphology to niche adaptation and pathogenesis is discussed, and the potential of morphological determinants as antimicrobial targets are examined.
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More than just lysins: peptidoglycan hydrolases tailor the cell wall
TL;DR: This review will focus on recent advances in this area with an emphasis on the regulation of PG hydrolases involved in cell division.
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Affinity of the New Cephalosporin CXA-101 to Penicillin-Binding Proteins of Pseudomonas aeruginosa
TL;DR: CXA-101 was found to be a potent PBP3 inhibitor and showed affinities ≥2-fold higher than those of ceftazidime for all of the essential PBPs (1b, 1c, 2, and 3).
Journal ArticleDOI
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis
Sophie Leclercq,Adeline Derouaux,Samir Olatunji,Claudine Fraipont,Alexander J. F. Egan,Waldemar Vollmer,Eefjan Breukink,Mohammed Terrak +7 more
TL;DR: It is found that FtsW, but not the other flippase candidate MurJ, impairs lipid II polymerization and peptide cross-linking activities of PBP1b, and that PBP3 relieves these inhibitory effects, suggesting a tight regulatory mechanism consistent with the cell’s need to ensure appropriate use of the limited pool of lipid II.
Journal ArticleDOI
The Peptidoglycan of Mycobacterium abscessus Is Predominantly Cross-Linked by l,d-Transpeptidases
Marie Lavollay,Martine Fourgeaud,Martine Fourgeaud,Martine Fourgeaud,Jean-Louis Herrmann,Lionel Dubost,Arul Marie,Laurent Gutmann,Michel Arthur,Michel Arthur,Michel Arthur,Jean-Luc Mainardi +11 more
TL;DR: It is shown that the peptidoglycans of the "rough" and "smooth" morphotypes contain predominantly 3→3 cross-links generated by l,d-transpeptidases, indicating that these enzymes are attractive targets for the development of efficient drugs.
References
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Peptidoglycan types of bacterial cell walls and their taxonomic implications.
Book
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TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.