The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
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TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
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Journal ArticleDOI
Development of a direct ELISA based on carboxy-terminal of penicillin-binding protein BlaR for the detection of β-lactam antibiotics in foods.
Juan Peng,Guyue Cheng,Lingli Huang,Yulian Wang,Haihong Hao,Dapeng Peng,Zhenli Liu,Zonghui Yuan +7 more
TL;DR: A rapid, simple, and accurate method for simultaneous detection of 15 β-lactams in edible tissues, among which 11 β- lactams controlled by European Union could be detected below maximum residue limits.
Journal ArticleDOI
Structure-Guided Design of Cell Wall Biosynthesis Inhibitors that Overcome Beta-Lactam Resistance in Staphylococcus Aureus (Mrsa).
Carlos Contreras-Martel,Ana Maria Amoroso,Esther C. Y. Woon,Astrid Zervosen,Steven R. Inglis,Alexandre Martins,Olivier Verlaine,Anna M. Rydzik,Viviane Job,André Luxen,Bernard Joris,Christopher J. Schofield,Andréa Dessen +12 more
TL;DR: This work demonstrates the potential of boronate-based PBP inhibitors for circumventing β-lactam resistance and opens avenues for the development of novel antibiotics that target Gram-positive pathogens.
Journal ArticleDOI
Single-molecule imaging reveals modulation of cell wall synthesis dynamics in live bacterial cells.
TL;DR: This work used single-molecule imaging to follow the behaviours of the two major peptidoglycan synthases in live, elongating Escherichia coli cells and after perturbation to suggest that multiple levels of regulation control the activity of enzymes to coordinate peptidlycercan synthesis.
Book ChapterDOI
The Murein Sacculus
Silke Litzinger,Christoph Mayer +1 more
TL;DR: The chemistry and composition of the murein as well as its biosynthesis and degradation will be covered, with a focus on Gram-negative bacteria; the autolytic system of Gram-positive bacteria is covered in a later chapter.
Journal ArticleDOI
Identification of pneumococcal proteins that are functionally linked to penicillin-binding protein 2b (PBP2b).
TL;DR: These findings suggest that DivIVA, which was previously assigned as a divisomal protein, is required to correctly localize the elongasome at the negatively curved membrane region between the septal and lateral cell wall.
References
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Peptidoglycan types of bacterial cell walls and their taxonomic implications.
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Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.