The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
Reads0
Chats0
TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
More filters
Metal nanostructures as antibacterial agents
TL;DR: In this chapter, several phenomena associated with cell damage will be explored and the influence of osmotic stress to the surface structure of bacterial cells cause pits and wrinkled protrusions, leading to increased permeability and cell death.
Journal ArticleDOI
Characterization of the elongasome core PBP2 : MreC complex of Helicobacter pylori.
Meriem El Ghachi,Pierre-Jean Matteï,Pierre-Jean Matteï,Chantal Ecobichon,Chantal Ecobichon,Alexandre Martins,Alexandre Martins,Sylviane Hoos,Sylviane Hoos,Christine Schmitt,Frédéric Colland,Christine Ebel,Christine Ebel,Marie-Christine Prévost,Frank Gabel,Frank Gabel,Patrick England,Patrick England,Andréa Dessen,Andréa Dessen,Ivo G. Boneca,Ivo G. Boneca +21 more
TL;DR: The specific expression in trans of the minimal interacting domain of MreC with PBP2 in the periplasmic space leads to cell rounding, suggesting that the P BP2/MreC complex formation in vivo is essential for cell morphology.
Journal ArticleDOI
Chemical Probes Reveal an Extraseptal Mode of Cross-Linking in Staphylococcus aureus
TL;DR: The results challenge the long-held belief that peptidoglycan synthesis is restricted to the septum in spherical bacteria, and instead indicate the presence of two spatiotemporally distinct modes of cross-linking in S. aureus: one at the sePTum during cell division, and another at the peripheral wall between divisions.
Journal ArticleDOI
A novel in vivo cell-wall labeling approach sheds new light on peptidoglycan synthesis in Escherichia coli.
Nick K. Olrichs,Mirjam E. G. Aarsman,Jolanda Verheul,Christopher J. Arnusch,Christopher J. Arnusch,Nathaniel I. Martin,Mireille Hervé,Waldemar Vollmer,Ben de Kruijff,Eefjan Breukink,Tanneke den Blaauwen +10 more
TL;DR: It is shown that Escherichia coli is able to import exogenous added murein tripeptide labeled with N‐7‐nitro‐2,1,3‐benzoxadiazol‐4‐yl (AeK–NBD) into the cytoplasm where it enters the peptidoglycan biosynthesis route, resulting in fluorescent labels specifically located in the cell wall.
Journal ArticleDOI
PBP 4 Mediates High-Level Resistance to New-Generation Cephalosporins in Staphylococcus aureus
Liana C. Chan,Aubre Gilbert,Li Basuino,Thaina Miranda da Costa,Thaina Miranda da Costa,Stephanie M. Hamilton,Katia Rn Dos Santos,Henry F. Chambers,Som S. Chatterjee +8 more
TL;DR: It is shown that S. aureus can also express high-level resistance to β-lactams, including new-generation broad-spectrum cephalosporins that are active against methicillin-resistant strains through an uncanonical core genome-encoded penicillin binding protein, PBP 4, a nonessential enzyme previously considered not to be important for staphylococcal β- lactam resistance.
References
More filters
Journal ArticleDOI
Peptidoglycan types of bacterial cell walls and their taxonomic implications.
Book
Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.