The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
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SurE is a trans-acting thioesterase cyclizing two distinct non-ribosomal peptides
Kenichi Matsuda,Masakazu Kobayashi,Takefumi Kuranaga,Kentaro Takada,Haruo Ikeda,Shigeki Matsunaga,Toshiyuki Wakimoto +6 more
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Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G
TL;DR: The feasibility to redirect the TCA cycle to drive a desired enzyme reaction, and this strategy could be applied to other enzymes that catalyze T CA cycle-coupleable reactions, is demonstrated.
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Promoting acid resistance and nisin yield of Lactococcus lactis F44 by genetically increasing D-Asp amidation level inside cell wall.
Panlong Hao,Dongmei Liang,Dongmei Liang,Lijie Cao,Lijie Cao,Bin Qiao,Bin Qiao,Hao Wu,Hao Wu,Qinggele Caiyin,Qinggele Caiyin,Hongji Zhu,Hongji Zhu,Jianjun Qiao,Jianjun Qiao +14 more
TL;DR: A novel strategy for enhancing nisin yield through cell wall remodeling is provided, which contributed to both continuous nisin synthesis and less nisin adsorption in acidic fermentation (dual enhancement).
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Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility.
Anissa D. Scholtzek,Dennis Hanke,Birgit Walther,Inga Eichhorn,Sabita D. Stöckle,Katja-Sophia Klein,Heidrun Gehlen,Antina Lübke-Becker,Stefan Schwarz,Andrea T. Feßler +9 more
TL;DR: Phenotypic resistance to penicillins, aminoglycosides, tetracyclines, fluoroquinolones and sulfamethoxazole/trimethoprim was observed in the respective isolates and β-lactamase activity was found to be associated with an inducible blaZ gene.
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High-Throughput Screening for Novel Inhibitors of Neisseria gonorrhoeae Penicillin-Binding Protein 2
Alena Fedarovich,Kevin A. Djordjevic,Shauna M. Swanson,Yuri K. Peterson,Robert A. Nicholas,Christopher Davies +5 more
TL;DR: A FP-based assay is developed that uses fluorescence polarization (FP) to find novel inhibitors of penicillin-binding proteins (PBPs) and paves the way for more comprehensive high-throughput screening against highly resistant strains of N. gonorrhoeae.
References
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