The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
TLDR
An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.Abstract:
Penicillin-binding proteins (PBPs) have been scrutinized for over 40 years. Recent structural information on PBPs together with the ongoing long-term biochemical experimental investigations, and results from more recent techniques such as protein localization by green fluorescent protein-fusion immunofluorescence or double-hybrid assay, have brought our understanding of the last stages of the peptidoglycan biosynthesis to an outstanding level that allows a broad outlook on the properties of these enzymes. Details are emerging regarding the interaction between the peptidoglycan-synthesizing PBPs and the peptidoglycan, their mesh net-like product that surrounds and protects bacteria. This review focuses on the detailed structure of PBPs and their implication in peptidoglycan synthesis, maturation and recycling. An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria.read more
Citations
More filters
Journal ArticleDOI
Evidence-based green algal genomics reveals marine diversity and ancestral characteristics of land plants
Marijke J. van Baren,Charles Bachy,Emily Nahas Reistetter,Samuel O. Purvine,Jane Grimwood,Sebastian Sudek,Hang Yu,Hang Yu,Camille Poirier,Thomas J. Deerinck,Alan Kuo,Igor V. Grigoriev,Chee Hong Wong,Richard D. Smith,Stephen J. Callister,Chia-Lin Wei,Jeremy Schmutz,Alexandra Z. Worden,Alexandra Z. Worden +18 more
TL;DR: These studies provide robust genomic resources for emerging model algae, advancing knowledge of marine phytoplankton and plant evolution, and indicate that peptidoglycan (PG) biosynthesis enzymes have been lost in multiple independent events in select prasinophytes and plants.
Journal ArticleDOI
Novel methicillin resistance gene mecD in clinical Macrococcus caseolyticus strains from bovine and canine sources
TL;DR: In this article, a methicillin-resistant Macrococcus caseolyticus strain from bovine and canine origins was found to carry a novel mecD gene conferring resistance to all classes of β-lactams including anti-MRSA cephalosporins.
Journal ArticleDOI
β-lactam Antibiotics Targeting PBP1 Selectively Enhance Daptomycin Activity against Methicillin-Resistant Staphylococcus aureus
TL;DR: This study examined the relative enhancement of DAP activity against MRSA by different β-lactam antibiotics to determine if a specific PBP-binding profile is associated with the ability to enhance the anti-MRSA activity, and found that both broad- and narrow-spectrum β- lactamiotics known to exhibit PBP1 binding demonstrated potent enhancement ofDAP anti- MRSA activity.
Journal ArticleDOI
Bacterial growth does require peptidoglycan hydrolases.
TL;DR: The identification in Escherichia coli of three new DD‐endopeptidases (Spr, YdhO and YebA) which are collectively required for peptidoglycan growth are reported, indicating that the cleavage of cross‐links by the new endopeptids is needed for surface growth of the sacculus.
Journal ArticleDOI
Ceftazidime-Avibactam: A Novel Cephalosporin/β-Lactamase Inhibitor Combination for the Treatment of Resistant Gram-negative Organisms
TL;DR: Ceftazidime, a third-generation cephalosporin when combined with avibactam has a significant improvement in its activity against β-lactamase-producing gram-negative pathogens, including extended-spectrum β- lactamases, AmpC β-bacteriaases, and Klebsiella pneumoniae carbapenemase- producing Enterobacteriaceae.
References
More filters
Journal ArticleDOI
Peptidoglycan types of bacterial cell walls and their taxonomic implications.
Book
Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
Journal ArticleDOI
Peptidoglycan structure and architecture
TL;DR: In several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions, and the different models for the architecture are discussed with respect to structural and physical parameters.
Journal ArticleDOI
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli
TL;DR: A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template.