Journal ArticleDOI
The protective role of glutathione in chlorothalonil-induced stoxicity to channel catfish
TLDR
The hypothesis that simultaneous exposure to multiple environmental chemicals that utilize or deplete tissue GSH may predis pose fish to acute toxicity is supported.About:
This article is published in Aquatic Toxicology.The article was published on 1992-09-01. It has received 99 citations till now. The article focuses on the topics: Catfish & Acute toxicity.read more
Citations
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Journal ArticleDOI
Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers
TL;DR: In this article, the effects of exposure to metals under and laboratory conditions were investigated in the Mediterranean mussel Mytilus galloprovincialis, including the concentrations of heavy metals, the level of glutathione, and the activity of several enzymes selected among glutathion-dependent oxidoreductases and hydrolases.
Journal ArticleDOI
A method to measure total antioxidant capacity against peroxyl radicals in aquatic organisms: application to evaluate microcystins toxicity.
Lílian Lund Amado,Márcia Longaray Garcia,Patrícia Baptista Ramos,Ricardo F. Freitas,Bruna Zafalon,Josencler L. Ribas Ferreira,João Sarkis Yunes,José Maria Monserrat +7 more
TL;DR: The use of antioxidant defenses was efficient in avoiding oxidative damage, as the content of oxidized proteins was not altered and the potential of this new methodology to be used in ecotoxicological studies is shown.
Journal ArticleDOI
Antioxidant responses and bioaccumulation in Ictalurus melas under mercury exposure
Antonia Concetta Elia,Roberta Galarini,Maria Illuminata Taticchi,Ambrosius Josef Martin Dörr,Luciana Mantilacci +4 more
TL;DR: The increase in the enzymes activities of catfish, involved in the inactivation of reactive molecules formed during oxidative stress, could provide an additional protection against the oxidative damage induced by mercury.
Journal ArticleDOI
Effects of Black Rock Harbor sediments on indices of biotransformation, oxidative stress, and DNA integrity in channel catfish
TL;DR: In this paper, a channel catfish (Ictalurus punctatus) was exposed in the laboratory to sediments obtained from either of two sites in Long Island Sound, CT.
Journal ArticleDOI
Effects of redox cycling compounds on glutathione content and activity of glutathione-related enzymes in rainbow trout liver.
TL;DR: Of the parameters included in the study, GR activity was the most responsive to treatment with redox cycling compounds, indicating that GR activity is a promising biomarker of such compounds and possibly indicating oxidative stress in rainbow trout.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine
TL;DR: It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione, and it is demonstrated that the total glutATHione concentration in mouse plasma is substantially higher than generally reported and that glutathion disulfide constitutes less than 30% of the totalglutathione present.
Book ChapterDOI
Determination of glutathione and glutathione disulfide in biological samples.
TL;DR: 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB)-GSSG reductase recycling assay for total glutathione is a specific, sensitive, rapid, and reliable procedure, however, because the method depends on an accurate standard curve, appropriate standards containing the protein precipitating agent are essential.
Journal ArticleDOI
Mechanism of action, metabolism, and toxicity of buthionine sulfoximine and its higher homologs, potent inhibitors of glutathione synthesis.
Journal ArticleDOI
Analysis of biological thiols: Derivatization with monobromobimane and separation by reverse-phase high-performance liquid chromatography
TL;DR: Since monobromobimane has little fluorescence and reacts very selectively with thiols to produce fluorescent derivatives, crude extracts can be derivatized and analyzed without prepurification of the thiolS, the entire process requiring only 1 to 2 h.