BookDOI
The protein protocols handbook
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Sectional Contents Only: Quantitation of Proteins and Peptides and Detection in Gels.Abstract:
Sectional Contents Only: Quantitation of Proteins. Electrophoresis of Proteins and Peptides and Detection in Gels. Blotting And Detection Methods. Chemical Modification of Proteins and Peptide Production and Purification. Protein/Peptide Characterisation. Glycoproteins. Immunochemical Techniques. Monoclonal Antibodies.read more
Citations
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Journal ArticleDOI
Reactive oxygen species, antioxidants, and the mammalian thioredoxin system.
Jonas Nordberg,Elias S.J. Arnér +1 more
TL;DR: The TrxR-catalyzed regeneration of several antioxidant compounds, including ascorbic acid (vitamin C), selenium-containing substances, lipoic acid, and ubiquinone are summarized.
Journal ArticleDOI
α-Synuclein in central nervous system and from erythrocytes, mammalian cells, and Escherichia coli exists predominantly as disordered monomer.
Bruno Fauvet,Martial K. Mbefo,Mohamed Bilal Fares,Carole Desobry,Sarah Michael,Mustafa T. Ardah,Elpida Tsika,Philippe Coune,Michel Prudent,Niels Lion,David Eliezer,Darren J. Moore,Bernard L. Schneider,Patrick Aebischer,Omar M. A. El-Agnaf,Eliezer Masliah,Hilal A. Lashuel +16 more
TL;DR: Wang et al. as discussed by the authors investigated the oligomeric state of α-syn in mouse, rat, and human brains and showed that both human and rodent αsyn expressed in the central nervous system exist predominantly as an unfolded monomer.
Journal ArticleDOI
Detection technologies in proteome analysis
TL;DR: New multiplexing capabilities should greatly enhance the applicability of the two-dimensional gel electrophoresis technique with respect to addressing fundamental questions related to proteome-wide changes in protein expression and post-translational modification.
Journal ArticleDOI
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
Maarten Fornerod,Jan M. van Deursen,Sjozef van Baal,Albert B. Reynolds,Donna S. Davis,K. Gopal Murti,Jack Fransen,Gerard Grosveld +7 more
TL;DR: The oncogenic nucleoporin CAN/Nup214 is essential in vertebrate cells and it is proposed that hCRM1 is a soluble nuclear transport factor that interacts with the NPC, which is a novel nuclear pore complex (NPC) component named Nup88.
Journal ArticleDOI
Protein adsorption in three dimensions.
TL;DR: The importance of this subject in biomaterials surface science is emphasized by reducing the "protein-adsorption problem" to three core questions that require quantitative answer, and several changes to the fundamental biophysical chemistry of protein adsorption are proposed.
References
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Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
TL;DR: Small amounts of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride membranes, stained with Coomassie Blue, and sequenced directly, suggesting that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
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A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids
D. Wessel,U.I. Flügge +1 more
TL;DR: A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed.
Journal ArticleDOI
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
TL;DR: With this apparatus, 50 protein bands from a human serum protein sample were detected by immunoblotting with the retainment of the high resolution of the SDS-PAGE technique.
Journal ArticleDOI
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
TL;DR: Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.