Journal ArticleDOI
The synthesis of 4-methylumbelliferyl α-ketoside of N-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase☆
Robert W. Myers,Reiko T. Lee,Yuan C. Lee,George H. Thomas,Linda W. Reynolds,Yoshihiro Uchida +5 more
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TLDR
4-Methylumbelliferyl α-ketoside of N- acetylneuraminic acid was synthesized by reacting the sodium salt of 4-methylumbelliferone with the 2-chloro-2-deoxy derivative of peracetylated methyl N-acetylNEuraminate, followed by preparative silica gel chromatography, deblocking, and purification by gel filtration on Sephadex G-25.About:
This article is published in Analytical Biochemistry.The article was published on 1980-01-01. It has received 160 citations till now. The article focuses on the topics: Sephadex & Neuraminidase.read more
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Book ChapterDOI
Chemistry, Metabolism, and Biological Functions of Sialic Acids
TL;DR: This chapter discusses the chemistry, metabolism, and biological functions of sialic acids and the biosynthesis of N-acetylneuraminic acid is briefly reported and more attention is given to the enzyme reactions modifying this compound.
Journal ArticleDOI
Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection.
Hope B. Tang,Emily DiMango,Ruth Bryan,M Gambello,Barbara H. Iglewski,Joanna B. Goldberg,Alice Prince +6 more
TL;DR: The expression of several P. aeruginosa virulence factors appears to be required to establish pulmonary infection in the neonatal mouse, as might be present in certain pathological conditions.
BookDOI
Biology of the sialic acids
TL;DR: Sialic Acid as Receptor Determinant of Ortho and Paramyxoviruses and the role of Gangliosides in Transmembrane Signaling and Cell Recognition is investigated.
Journal ArticleDOI
Enzyme Immunoassays for the Detection of Infectious Antigens in Body Fluids: Current Limitations and Future Prospects
TL;DR: Standard enzyme immunoassay techniques are not sufficiently sensitive for the measurement of some antigens from other viruses, bacteria, and parasites in concentrations that commonly occur in body fluids during the course of infectious diseases.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
Journal ArticleDOI
Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.
TL;DR: Neuraminidase activity was considerably reduced in cultured fibroblasts of patients with mucolipidosis types I, II, and III and in cultured Fibroblast and leucocyte neuraminidases showed maximum activity between pH 4.2 and 4.4.
Journal ArticleDOI
Synthese anomerer Sialinsäure-methylketoside
TL;DR: In this article, the Acetochlor-Verbindung des Lactaminsaure-methylesters wurde nach Koenigs-Knorr ein Methylketosidmethylter von [α]: −6° erhalten, der von Neuraminidase quantitativ gespalten wird.
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Rational design of potent sialidase-based inhibitors of influenza virus replication.
Mark von Itzstein,Wen-Yang Wu,Gaik B. Kok,Michael S. Pegg,Jeffrey Clifford Dyason,Betty Jin,Tho Van Phan,Mark L. Smythe,Hume Forrest White,Hume Forrest White,Stuart W. Oliver,Peter M. Colman,Joseph N. Varghese,D. Michael Ryan,Jacqueline M. Woods,Richard C. Bethell,Vanessa J. Hotham,Janet M. Cameron,Charles R. Penn +18 more