The vaginal microbiome during pregnancy and the postpartum period in a European population
David A. MacIntyre,Manju Chandiramani,Yun S. Lee,L Kindinger,Ann Smith,Nicos Angelopoulos,Benjamin Lehne,Shankari Arulkumaran,Richard J. C. Brown,T. G. Teoh,Elaine Holmes,Jeremy K. Nicoholson,Julian R. Marchesi,Phillip R. Bennett +13 more
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TLDR
It is shown that vaginal microbiome composition dramatically changes postpartum to become less Lactobacillus spp.Abstract:
The composition and structure of the pregnancy vaginal microbiome may influence susceptibility to adverse pregnancy outcomes. Studies on the pregnant vaginal microbiome have largely been limited to Northern American populations. Using MiSeq sequencing of 16S rRNA gene amplicons, we characterised the vaginal microbiota of a mixed British cohort of women (n = 42) who experienced uncomplicated term delivery and who were sampled longitudinally throughout pregnancy (8–12, 20–22, 28–30 and 34–36 weeks gestation) and 6 weeks postpartum. We show that vaginal microbiome composition dramatically changes postpartum to become less Lactobacillus spp. dominant with increased alpha-diversity irrespective of the community structure during pregnancy and independent of ethnicity. While the pregnancy vaginal microbiome was characteristically dominated by Lactobacillus spp. and low alpha-diversity, unlike Northern American populations, a significant number of pregnant women this British population had a L. jensenii-dominated microbiome characterised by low alpha-diversity. L. jensenii was predominantly observed in women of Asian and Caucasian ethnicity whereas L. gasseri was absent in samples from Black women. This study reveals new insights into biogeographical and ethnic effects upon the pregnancy and postpartum vaginal microbiome and has important implications for future studies exploring relationships between the vaginal microbiome, host health and pregnancy outcomes.read more
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DADA2: High-resolution sample inference from Illumina amplicon data
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TL;DR: How prenatal and postnatal factors shape the development of both the microbiome and the immune system are described and the prospects of microbiome-mediated therapeutics and the need for more effective approaches that can reconfigure bacterial communities from pathogenic to homeostatic configurations are discussed.
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The vaginal microbiome and preterm birth
Jennifer M. Fettweis,Myrna G. Serrano,J. Paul Brooks,David J. Edwards,Philippe H. Girerd,Hardik I. Parikh,Bernice Huang,Tom Arodz,Laahirie Edupuganti,Abigail L. Glascock,Jie Xu,Nicole R. Jimenez,Stephany C. Vivadelli,Stephen S. Fong,Nihar U. Sheth,Sophonie Jean,Vladimir Lee,Yahya Bokhari,Ana M. Lara,Shreni D. Mistry,Robert A. Duckworth,Steven P. Bradley,Vishal N. Koparde,X. Valentine Orenda,Sarah Milton,Sarah K. Rozycki,Andrey V. Matveyev,Michelle L. Wright,Michelle L. Wright,Snehalata Huzurbazar,Eugenie M. Jackson,Ekaterina Smirnova,Ekaterina Smirnova,Jonas Korlach,Yu-Chih Tsai,Molly R. Dickinson,Jamie L. Brooks,Jennifer I. Drake,Donald O. Chaffin,Amber L. Sexton,Michael G. Gravett,Craig E. Rubens,N. Romesh Wijesooriya,Karen D. Hendricks-Muñoz,Kimberly K. Jefferson,Jerome F. Strauss,Gregory A. Buck +46 more
TL;DR: A community resource that includes ‘omics’ data from approximately 12,000 samples as part of the integrative Human Microbiome Project is reported, identifying harbingers of preterm birth in this cohort of women predominantly of African ancestry.
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