Transendocardial, Autologous Bone Marrow Cell Transplantation for Severe, Chronic Ischemic Heart Failure
Emerson C. Perin,Hans F. Dohmann,Radovan Borojevic,Suzana A. Silva,Andre L.S. Sousa,Claudio Tinoco Mesquita,Maria Isabel D. Rossi,Antonio Carlos Carvalho,Hélio S. Dutra,Hans J.F. Dohmann,Guilherme V. Silva,Luciano Belem,Ricardo Vivacqua,Fernando Oswaldo Dias Rangel,Roberto Esporcatte,Yong-Jian Geng,William K. Vaughn,Joao A.R. Assad,Evandro Tinoco Mesquita,James T. Willerson +19 more
Reads0
Chats0
TLDR
The present study demonstrates the relative safety of intramyocardial injections of bone marrow–derived stem cells in humans with severe heart failure and the potential for improving myocardial blood flow with associated enhancement of regional and global left ventricular function.Abstract:
Background— This study evaluated the hypothesis that transendocardial injections of autologous mononuclear bone marrow cells in patients with end-stage ischemic heart disease could safely promote neovascularization and improve perfusion and myocardial contractility. Methods and Results— Twenty-one patients were enrolled in this prospective, nonrandomized, open-label study (first 14 patients, treatment; last 7 patients, control). Baseline evaluations included complete clinical and laboratory evaluations, exercise stress (ramp treadmill), 2D Doppler echocardiogram, single-photon emission computed tomography perfusion scan, and 24-hour Holter monitoring. Bone marrow mononuclear cells were harvested, isolated, washed, and resuspended in saline for injection by NOGA catheter (15 injections of 0.2 cc). Electromechanical mapping was used to identify viable myocardium (unipolar voltage ≥6.9 mV) for treatment. Treated and control patients underwent 2-month noninvasive follow-up, and treated patients alone underwen...read more
Citations
More filters
Journal ArticleDOI
Delivery and tracking of therapeutic cell preparations for clinical cardiovascular applications.
R De Silva,Robert J. Lederman +1 more
TL;DR: A brief review of experimental observations suggesting adult stem cell plasticity and cross-lineage transdifferentiation in cell therapy for cardiovascular disease, with emphasis on the choice of cell preparation, route of cell delivery and tracking of delivered cells.
Journal ArticleDOI
Diabetic and Nondiabetic Patients Respond Differently to Transendocardial Injection of Bone Marrow Mononuclear Cells: Findings From Prospective Clinical Trials in ''No-Option'' Patients
Pilar Jiménez-Quevedo,Guillherme V. Silva,Ricardo Sanz-Ruiz,Edie M. Oliveira,Marlos R. Fernandes,Franca Angeli,James T. Willerson,Hans F. Dohmann,Emerson C. Perin +8 more
TL;DR: This is the first clinical study to show that BMMNC injection could have a smaller effect in diabetics, and there was a significant decrease in endsystolic volume between baseline and 6-month follow-up.
Journal ArticleDOI
Cellular cardiomyoplasty in large myocardial infarction : Can the beneficial effect be enhanced by ACE-inhibitor therapy?
Emerson L. Olivares,Ricardo H. Costa-e-Sousa,Joao Pedro Werneck-de-Castro,Vanessa Pinho-Ribeiro,Marcia Gracindo da Silva,Karla Consort Ribeiro,Elisabete C. Mattos,Regina Coeli dos Santos Goldenberg,Antonio Carlos Carvalho,Antonio Carlos Carvalho,Masako Oya Masuda +10 more
TL;DR: The hypothesis that captopril treatment could enhance the beneficial effect of cell therapy in large myocardial infarctions in patients with infarcted hearts is tested.
Journal ArticleDOI
Stem cell therapy for heart failure.
TL;DR: The primary mechanism of action for cell therapy is now believed to be through paracrine effects that include the release of cytokines, chemokines, and growth factors that inhibit apoptosis and fibrosis, enhance contractility, and activate endogenous regenerative mechanisms through endogenous circulating or site-specific stem cells.
Journal ArticleDOI
Exogenous bone marrow cells do not rescue non-irradiated mice from acute renal tubular damage caused by HgCl2, despite establishment of chimaerism and cell proliferation in bone marrow and spleen.
T.-C. Fang,William R. Otto,Rosemary Jeffery,Tim Hunt,M. R. Alison,H. T. Cook,Nicholas A. Wright,R. Poulsom +7 more
TL;DR: This is the first report to show that despite no preparative myeloablation of recipients, engrafted donor BMCs can synthesize DNA in the bone marrow and spleen and display proliferative activity.
References
More filters
Journal ArticleDOI
Isolation of putative progenitor endothelial cells for angiogenesis.
Takayuki Asahara,Toyoaki Murohara,Alison Sullivan,Marcy Silver,Rien van der Zee,Tong Li,Bernhard Witzenbichler,Gina C. Schatteman,Jeffrey M. Isner +8 more
TL;DR: It is suggested that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarianAngiogenesis.
Journal ArticleDOI
Bone marrow cells regenerate infarcted myocardium
Donald Orlic,Jan Kajstura,Stefano Chimenti,Igor Jakoniuk,Stacie M. Anderson,Baosheng Li,James Pickel,Ronald D.G. McKay,Bernardo Nadal-Ginard,David M. Bodine,Annarosa Leri,Piero Anversa +11 more
TL;DR: It is indicated that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.
Journal ArticleDOI
Neovascularization of ischemic myocardium by human bone-marrow–derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function
Alfred Kocher,Michael D. Schuster,Matthias Szabolcs,Shin Takuma,Daniel Burkhoff,Jennifer Wang,Shunichi Homma,Niloo M. Edwards,Silviu Itescu +8 more
TL;DR: It is shown that bone marrow from adult humans contains endothelial precursors with phenotypic and functional characteristics of embryonic hemangioblasts, and that these can be used to directly induce new blood vessel formation in the infarct-bed and proliferation of preexisting vasculature after experimental myocardial infarction.
Journal ArticleDOI
ACC/AHA 2002 Guideline Update for Exercise Testing: Summary Article A Report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Committee to Update the 1997 Exercise Testing Guidelines)
Raymond J. Gibbons,Gary J. Balady,J. Timothy Bricker,Bernard R. Chaitman,Gerald F. Fletcher,Victor F. Froelicher,Daniel B. Mark,Ben D. McCallister,Aryan N. Mooss,Michael G. O’Reilly,William L. Winters,Elliott M. Antman,Joseph S. Alpert,David P. Faxon,Valentin Fuster,Gabriel Gregoratos,Loren F. Hiratzka,Alice K. Jacobs,Richard O. Russell,Sidney C. Smith +19 more
TL;DR: The American College of Cardiology (ACC)/AHA Task Force on Practice Guidelines regularly reviews existing guidelines to determine when an update or full revision is needed.
Journal ArticleDOI
Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine Heart
TL;DR: The persistence of the engrafted hMSCs and their in situ differentiation in the heart may represent the basis for using these adult stem cells for cellular cardiomyoplasty.