Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.
Jean Weissenbach,Yuti Chernajovsky,Menachem Zeevi,Lester M. Shulman,Hermona Soreq,Uri Nir,David Wallach,Michel Perricaudet,Pierre Tiollais,M Revel +9 more
TLDR
Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322, but both are induced by poly(rI.rC), but IFN-beta 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFn-beta 1 is not induced.Abstract:
Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-beta 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mr protein. The second interferon mRNA species (Hu IFN-beta 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI.rC), but IFN-beta 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-beta 1 is not induced.read more
Citations
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The biology of interleukin-6.
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Interleukin-6 in biology and medicine
TL;DR: Application of IL-6 is promising in cancer treatment as well as in treatment of radiation- or chemotherapy-induced myelosuppression and the cell biology of the intracellular events that link transduction to gene regulation is an important area, and work on these topics helps to understand such phenomena as multifunction of IL -6 and bidirectional effects of cell growth depending on the cell type.
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