Journal ArticleDOI
Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies
TLDR
In this paper, the enzyme-strand recycling and hairpin catalytic assembly (HCA) reaction were used as dual enzyme-free amplification strategies for detection of uranyl in river waters samples.Abstract:
A novel fluorescent and colorimetric strategy with enzyme-free dual amplification was developed for ultra-sensitively detection of uranyl in river waters samples. The enzyme-strand recycling and hairpin catalytic assembly (HCA) reaction were used as dual enzyme-free amplification strategies. Three hairpins labeled with fluorophore can absorb on the surface of gold nanoparticles (AuNPs) to prevent them from aggregation in salt solution and quench the fluorescent signal simultaneously. In the presence of UO22+, the HCA reaction between hairpins was induced to form rigid DNA triangles, resulting in the aggregation of AuNPs with a red to blue color change and a “turn-on” fluorescent signal. The limit of detection was as low as 0.1 pM. Importantly, this strategy provided colorimetric and fluorescent dual signals for lower false-positives and higher anti-interference ability. It was successfully used for detection of uranyl in real waters samples.read more
Citations
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Biosensing with DNAzymes
TL;DR: A comprehensive review of biosensing with DNAzymes, providing an overview of different sensing applications while highlighting major progress and seminal contributions to the field of portable biosensor devices and point-of-care diagnostics is provided in this paper.
Journal ArticleDOI
Sensors for determination of uranium: A review
Xumeng Wu,Qiuxiang Huang,Yu Mao,Xiangxue Wang,Yuyuan Wang,Qinghua Hu,Hongqing Wang,Xiangke Wang,Xiangke Wang +8 more
TL;DR: In this paper, a review summarizes relevant literature on the detection of uranyl ions by different sensors over the years, which can be roughly divided into three categories: optical sensors, electrochemical sensors and other sensors.
Journal ArticleDOI
Programmable DNA switches and their applications
Scott G. Harroun,Carl Prévost-Tremblay,Dominic Lauzon,Arnaud Desrosiers,Xiaomeng Wang,Liliana Pedro,Alexis Vallée-Bélisle +6 more
TL;DR: This review discusses how to engineer DNA switches starting from the selection of a specific DNA-based recognition element, to its adaptation and optimisation into a switch, with applications ranging from sensing to drug delivery, smart materials, molecular transporters, logic gates and others.
Journal ArticleDOI
A novel nest hybridization chain reaction based electrochemical assay for sensitive detection of circulating tumor DNA.
Yifang Huang,Maliang Tao,Shihua Luo,Ye Zhang,Bo Situ,Xinyi Ye,PeiWen Chen,Xiujuan Jiang,Qian Wang,Lei Zheng +9 more
TL;DR: A novel electrochemical biosensor based on nest hybridization chain reaction was proposed for the sensitive and specific detection of PIK3CA E545K ct DNA with a simple process and successfully applied to the detection of ctDNA in spiked-in samples, pleural effusion and serum samples of malignant tumor patients.
Journal ArticleDOI
Porphyrinic Metal-Organic Framework Nanorod-Based Dual-Modal Nanoprobe for Sensing and Bioimaging of Phosphate.
Changming Cheng,Changming Cheng,Ruolin Zhang,Jiuhai Wang,Yu Zhang,Shunshun Xiong,Ying Huang,Mo Yang +7 more
TL;DR: Benefiting from the good biocompatibility and water dispersibility of PCN-222 NRs, this nanoprobe was successfully employed in the intracellular imaging of phosphate, revealing its promising application in the biological science.
References
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Journal ArticleDOI
Programming biomolecular self-assembly pathways
TL;DR: Diverse molecular self-assembly and disassembly pathways are program using a ‘reaction graph’ abstraction to specify complementarity relationships between modular domains in a versatile DNA hairpin motif.
Journal ArticleDOI
Rolling circle amplification: A versatile tool for chemical biology, materials science and medicine
M. Monsur Ali,Feng Li,Feng Li,Zhiqing Zhang,Zhiqing Zhang,Kaixiang Zhang,Kaixiang Zhang,Dong-Ku Kang,James A. Ankrum,X. Chris Le,Weian Zhao +10 more
TL;DR: The fundamental engineering principles used to design RCA nanotechnologies are introduced, the recently developed RCA-based diagnostics and bioanalytical tools are discussed, and the use of RCA to construct multivalent molecular scaffolds and nanostructures for applications in biology, diagnostic and therapeutics is summarized.
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Properties, use and health effects of depleted uranium (DU): a general overview.
A. Bleise,P.R. Danesi,W Burkart +2 more
TL;DR: Depleted uranium, a waste product of uranium enrichment, has several civilian and military applications and was used as armor-piercing ammunition in international military conflicts and was claimed to contribute to health problems, known as the Gulf War Syndrome and recently as the Balkan Syndrome.
Journal ArticleDOI
Visual Detection of Copper(II) by Azide- and Alkyne-Functionalized Gold Nanoparticles Using Click Chemistry
TL;DR: This method allows the naked eye, without the aid of any advanced instrument, to assay for the presence of Cu ions by the aggregation of Au NPs as a result of the Cu(I)-catalyzed conjugation between the two functional groups.